Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish

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	Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish
	Feng, Yan ; Yan, Tingting ; Zheng, Jin ; Ge, Xinjian ; Mu, Yu ; Zhang, Yi ; Wu, Dongmei ; Du, Jiu-lin ; Zhai, Qiwei
刊名	JOURNAL OF NEUROSCIENCE RESEARCH
	2010
卷号	88 期号:15 页码:3319-3327
关键词	Nmnat2 Wallerian degeneration axon degeneration neurodegeneration NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE WALLERIAN DEGENERATION IN-VIVO ADENINE-DINUCLEOTIDE ENZYMATIC-ACTIVITY NAD BIOSYNTHESIS MODEL SLOW GENE REGENERATION
ISSN号	0360-4012
通讯作者	Zhai, QW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab, 294 Tai Yuan Rd, Shanghai 200031, Peoples R China,qwzhai@sibs.ac.cn
英文摘要	Axon degeneration is supposed to be a therapeutic target for treating neurodegenerative diseases. Mauthner cells (M-cells) are ideal for studying axons in vivo because of their limited numbers, large size, and long axons. In this study, we labeled M-cells by single-cell electroporation with plasmids expressing DsRed2 or EGFP. Injury-induced axon degeneration in labeled M-cell was imaged under a confocal microscope, and we found that the Mauthner axons started to degenerate about 24 hr after lesion. The Wld(S) protein containing full-length Nmnat1 is well-known for its axon-protective function in many systems. Overexpression of Wlds in M-cells also greatly delayed axon degeneration in live zebrafish. Nmnat2 is the only Nmnat highly expressed in brain. Here we demonstrated that overexpression of Nmnat2 in M-cells significantly delayed axon degeneration in vivo, and disruption of the NAD synthesis activity of Nmnat2 markedly attenuated its axon-protective function. All these data show that injury-induced axon degeneration of M-cell has a mechanism similar to that in mammalians and would be a valuable model for studying axon degeneration in vivo. (C) 2010 Wiley-Liss, Inc.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	National Natural Science Foundation of China[30825009, 30970619]; National Basic Research Program of China (973 Program)[2009CB918403, 2006CB943802, 2007CB914501]; Science and Technology Commission of Shanghai Municipality[07DJ14005, 08QH1402600]; Shanghai Institutes for Biological Sciences[2007KIP103]; Chinese Academy of Sciences
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1565]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Feng, Yan,Yan, Tingting,Zheng, Jin,et al. Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2010,88(15):3319-3327.
APA	Feng, Yan.,Yan, Tingting.,Zheng, Jin.,Ge, Xinjian.,Mu, Yu.,...&Zhai, Qiwei.(2010).Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish.JOURNAL OF NEUROSCIENCE RESEARCH,88(15),3319-3327.
MLA	Feng, Yan,et al."Overexpression of Wld(S) or Nmnat2 in Mauthner Cells by Single-Cell Electroporation Delays Axon Degeneration in Live Zebrafish".JOURNAL OF NEUROSCIENCE RESEARCH 88.15(2010):3319-3327.