NeuroD2 regulates the development of hippocampal mossy fiber synapses

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	NeuroD2 regulates the development of hippocampal mossy fiber synapses
	Qiu, Zilong
刊名	NEURAL DEVELOPMENT
	2012
卷号	7 页码:41161
关键词	ACTIVITY-DEPENDENT REGULATION MAGUK SCAFFOLDING PROTEINS LONG-TERM POTENTIATION SYNAPTIC PLASTICITY DENDRITIC SPINES IN-VIVO POSTNATAL-DEVELOPMENT RAT HIPPOCAMPUS CRITICAL PERIOD AMPA RECEPTORS
ISSN号	1749-8104
通讯作者	Ghosh, A (reprint author), Univ Calif San Diego, Neurobiol Sect, Div Biol Sci, San Diego, CA 92093 USA,aghosh@ucsd.edu
英文摘要	Background: The assembly of neural circuits requires the concerted action of both genetically determined and activity-dependent mechanisms. Calcium-regulated transcription may link these processes, but the influence of specific transcription factors on the differentiation of synapse-specific properties is poorly understood. Here we characterize the influence of NeuroD2, a calcium-dependent transcription factor, in regulating the structural and functional maturation of the hippocampal mossy fiber (MF) synapse. Results: Using NeuroD2 null mice and in vivo lentivirus-mediated gene knockdown, we demonstrate a critical role for NeuroD2 in the formation of CA3 dendritic spines receiving MF inputs. We also use electrophysiological recordings from CA3 neurons while stimulating MF axons to show that NeuroD2 regulates the differentiation of functional properties at the MF synapse. Finally, we find that NeuroD2 regulates PSD95 expression in hippocampal neurons and that PSD95 loss of function in vivo reproduces CA3 neuron spine defects observed in NeuroD2 null mice. Conclusion: These experiments identify NeuroD2 as a key transcription factor that regulates the structural and functional differentiation of MF synapses in vivo.
学科主题	Developmental Biology ; Neurosciences & Neurology
收录类别	SCI
资助信息	[MH068578]; [NS064124]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1498]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_神经可塑性分子基础研究组
推荐引用方式 GB/T 7714	Qiu, Zilong. NeuroD2 regulates the development of hippocampal mossy fiber synapses[J]. NEURAL DEVELOPMENT,2012,7:41161.
APA	Qiu, Zilong.(2012).NeuroD2 regulates the development of hippocampal mossy fiber synapses.NEURAL DEVELOPMENT,7,41161.
MLA	Qiu, Zilong."NeuroD2 regulates the development of hippocampal mossy fiber synapses".NEURAL DEVELOPMENT 7(2012):41161.