K+ regulates DNA binding of transcription factors to control gene expression related to neuronal apoptosis

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	K+ regulates DNA binding of transcription factors to control gene expression related to neuronal apoptosis
	Wang, Yizheng
刊名	NEUROREPORT
	2006
卷号	17 期号:11 页码:1199-1204
关键词	cAMP-responsive element-binding protein DNA binding Forkhead neuron apoptosis p53 potassium SYMPATHETIC NEURONS CELL-SURVIVAL ACTIVATION POTASSIUM CREB DEATH MEDIATION PROTEINS KINASE
ISSN号	0959-4965
通讯作者	Wang, YZ (reprint author), Chinese Acad Sci, Inst Neurosci, SIBS, Lab Neural Signal Transduct,Grad Sch, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,yzwang@ion.ac.cn
英文摘要	The loss of intracellular K+ promotes neuronal apoptosis. The mechanism by which K+ acts on apoptosis, however, remains largely unknown. Here we showed that K+ selectively affects DNA binding activity of transcriptional factors in vitro. Low K+ concentration ([K+]) promoted the DNA binding activity of p53 and Forkhead, proapoptotic transcriptional factors, whereas it inhibited that of cAMP-responsive element-binding protein, an anti-apoptotic transcriptional factor. In contrast, K+ did not affect the DNA binding activity of Ying Yang 1, CCAAT/enhancer binding protein and early growth response protein-L The expression of bax and bim, proapoptotic genes known to be regulated by pS3 and Forkhead, respectively, was enhanced in cortical neurons deprived of serum, condition known to cause K+ loss, whereas the expression of cfos, a cAMP-responsive element-binding protein target gene, was inhibited. Furthermore, blocking K+ channels suppressed the enhancement of bim mRNA level and the reduction of c-fos mRNA level induced by K+ loss, whereas it had no effect on the stimulation of Forkhead or cAMP-responsive element-binding protein induced by K+ loss. These results suggest that low intracellular [K+] selectively affects DNA binding activity of transcriptional factors to regulate gene expression related to neuronal apoptosis.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1810]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_神经信号转导研究组
推荐引用方式 GB/T 7714	Wang, Yizheng. K+ regulates DNA binding of transcription factors to control gene expression related to neuronal apoptosis[J]. NEUROREPORT,2006,17(11):1199-1204.
APA	Wang, Yizheng.(2006).K+ regulates DNA binding of transcription factors to control gene expression related to neuronal apoptosis.NEUROREPORT,17(11),1199-1204.
MLA	Wang, Yizheng."K+ regulates DNA binding of transcription factors to control gene expression related to neuronal apoptosis".NEUROREPORT 17.11(2006):1199-1204.