Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone

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	Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone
	Xiong, Zhi-Qi
刊名	CELL RESEARCH
	2011
卷号	21 期号:2 页码:338-349
关键词	adult neurogenesis doublecortin genetic labeling dentate gyrus spine NEWLY GENERATED NEURONS ENHANCED SYNAPTIC PLASTICITY CENTRAL-NERVOUS-SYSTEM HILAR MOSSY CELLS DENTATE GYRUS GRANULE CELLS TRANSGENIC MICE PSA-NCAM NEUROGENESIS HIPPOCAMPUS
ISSN号	1001-0602
通讯作者	Xiong, ZQ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Inst Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China,xiongzhiqi@ion.ac.cn
英文摘要	Research over the past decades has demonstrated that adult brain produces neural progenitor cells which proliferate and differentiate to newborn neurons that integrate into the existing circuit. However, detailed differentiation processes and underlying mechanisms of newly generated neurons are largely unknown due to the limitation of available methods for labeling and manipulating neural progenitor cells and newborn neurons. In this study, we designed a tightly controlled, noninvasive system based on Cre/loxP recombination to achieve long-term tracing and genetic manipulation of adult neurons in vivo. In this system, tamoxifen-inducible recombinase, CreER(T2), was driven by BAC-based promoter of doublecortin (DCX, a marker of newborn neurons). By crossing this Cre line with reporter mouse, we found that newborn neurons in the dentate gyrus (DG) could be selectively pulse-labeled by tamoxifen-induced expression of yellow fluorescent protein (YFP). YFP-positive neurons were identified by coimmunostaining with cell type-specific markers and characterized by electrophysiological recording. Furthermore, analysis of the migration of these neurons showed that the majority of these labeled neurons migrated to the inner part of granule cell layer. Moreover, spine growth of inner molecular layer of newborn granule neurons takes a dynamic pattern of invert U-shape, in contrast to the wedge-shaped change in the outer molecular layer. Our transgenic tool provides an efficient way to selectively label and manipulate newborn neuron in adult mouse DG.
学科主题	Cell Biology
收录类别	SCI
资助信息	Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences[SIBS2008003]; National Basic Research Program of China[2006CB806600]; Key State Research Program of China[2006CB943900]; Innovative Research Group of the National Natural Science Foundation of China[30721004]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1554]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_疾病神经生物学研究组
推荐引用方式 GB/T 7714	Xiong, Zhi-Qi. Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone[J]. CELL RESEARCH,2011,21(2):338-349.
APA	Xiong, Zhi-Qi.(2011).Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone.CELL RESEARCH,21(2),338-349.
MLA	Xiong, Zhi-Qi."Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone".CELL RESEARCH 21.2(2011):338-349.