Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway

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	Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway
	Li, Shun; Sun, Fan; Zhang, Yi-Bing; Gui, Jian-Fang; Zhang, Qi-Ya
刊名	PLOS ONE
	2012-03-07
卷号	7 期号:3 页码:e32427
关键词	HEPATITIS-C-VIRUS TROUT ONCORHYNCHUS-MYKISS DEPENDENT PROTEIN-KINASE IFN-BETA PROMOTER I-LIKE RECEPTORS RIG-I FUNCTIONAL-CHARACTERIZATION PARALICHTHYS-OLIVACEUS SPRING VIREMIA CARP VIRUS
ISSN号	1932-6203
通讯作者	Li, S (reprint author), Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China.
中文摘要	Background: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) had been demonstrated to prime interferon (IFN) response against viral infection via the conserved RLR signaling in fish, and a novel fish-specific gene, the grass carp reovirus (GCRV)-induced gene 2 (Gig2), had been suggested to play important role in host antiviral response. Methodology/Principal Findings: In this study, we cloned and characterized zebrafish Gig2 homolog (named Danio rerio Gig2-I, DreI), and revealed its antiviral role and expressional regulation signaling pathway. RT-PCR, Western blot and promoter activity assay indicate that DreI can be induced by poly I: C, spring viremia of carp virus (SVCV) and recombinant IFN (rIFN), showing that DreI is a typical ISG. Using the pivotal signaling molecules of RLR pathway, including RIG-I, MDA5 and IRF3 from crucian carp, it is found that DreI expression is regulated by RLR cascade and IRF3 plays an important role in this regulation. Furthermore, promoter mutation assay confirms that the IFN-stimulated regulatory elements (ISRE) in the 59 flanking region of DreI is essential for its induction. Finally, overexpression of DreI leads to establish a strong antiviral state against SVCV and Rana grylio virus (RGV) infection in EPC (Epithelioma papulosum cyprinid) cells. Conclusions/Significance: These data indicate that DreI is an antiviral protein, which is regulated by RLR signaling pathway.
英文摘要	Background: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) had been demonstrated to prime interferon (IFN) response against viral infection via the conserved RLR signaling in fish, and a novel fish-specific gene, the grass carp reovirus (GCRV)-induced gene 2 (Gig2), had been suggested to play important role in host antiviral response.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	HEPATITIS-C-VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; DEPENDENT PROTEIN-KINASE ; IFN-BETA PROMOTER ; I-LIKE RECEPTORS ; RIG-I ; FUNCTIONAL-CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; CARP VIRUS
收录类别	SCI
资助信息	National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16]
语种	英语
WOS记录号	WOS:000303060800019
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16947]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Li, Shun,Sun, Fan,Zhang, Yi-Bing,et al. Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway[J]. PLOS ONE,2012,7(3):e32427.
APA	Li, Shun,Sun, Fan,Zhang, Yi-Bing,Gui, Jian-Fang,&Zhang, Qi-Ya.(2012).Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway.PLOS ONE,7(3),e32427.
MLA	Li, Shun,et al."Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway".PLOS ONE 7.3(2012):e32427.