Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus
	Yang, Yuhui 1; Peng, Kou 1; Liu, Xiaoyan 1; Zhao, Dongmei 1; Duan, Jundan 1; Hu, Wei 2; Zhao, Haobin 1
刊名	AQUACULTURE
	2012-06-20
卷号	350 页码:1-7
关键词	Gobiocypris rarus Myostatin Tempo-spatial expression Real time PCR 17 alpha-ethynyl estradiol (EE2) 17 alpha-methyl testosterone (MT)
ISSN号	0044-8486
通讯作者	Zhao, HB (reprint author), Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
中文摘要	Myostatin (MSTN), belonging to transforming growth factor (TGF) beta superfamily, is a negative regulator of muscular development in mammals and fish. The expression of MSTN can be regulated by hormone and environmental factors. Sex steroids, androgen and estrogen can affect sex differentiation and also can affect muscle development. However, few documents are concerned about the effects of sex steroids on MSTN expression in fish. Here, we report the cloning and expressional analyses of MSTN in rare minnow (Gobiocypris rarus), an emerging model fish in China. The MSTN cDNA of rare minnow that has an open reading frame of 1128 base pairs (bp) encodes a protein of 375 amino acids belonging to fish MSTN1 with typical structures like a prodomain, a mature MSTN domain, a conservative hydrolytic site (RIRR) and 9 conservative cysteine residues in mature MSTN. MSTN was detectable in all the examined tissues by RT-PCR that indicated the ubiquitous expression of MSTN in adult tissues. MSTN was detectable from zygote till hatching showed the maternal deposition of MSTN mRNA from oocytes and continuous expression during embryogenesis. Treatment of rare minnow with 1 mu g/L 17 alpha-methyl testosterone (MT) or 17 alpha-ethynyl estradiol (EE2) significantly impaired the body growth and increased the expression of MSTN mRNA detected by quantitative real time PCR. However, low dosage of MT (10 ng/L) had no significant effects on body growth and MSTN expression. The results suggest that the effects of exogenous sex steroids on growth can be mediated through MSTN. It is also suggested that low concentration of sex steroid (less than 10 ng/L) may have little effect on MSTN expression. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要	Myostatin (MSTN), belonging to transforming growth factor (TGF) beta superfamily, is a negative regulator of muscular development in mammals and fish. The expression of MSTN can be regulated by hormone and environmental factors. Sex steroids, androgen and estrogen can affect sex differentiation and also can affect muscle development. However, few documents are concerned about the effects of sex steroids on MSTN expression in fish. Here, we report the cloning and expressional analyses of MSTN in rare minnow (Gobiocypris rarus), an emerging model fish in China. The MSTN cDNA of rare minnow that has an open reading frame of 1128 base pairs (bp) encodes a protein of 375 amino acids belonging to fish MSTN1 with typical structures like a prodomain, a mature MSTN domain, a conservative hydrolytic site (RIRR) and 9 conservative cysteine residues in mature MSTN. MSTN was detectable in all the examined tissues by RT-PCR that indicated the ubiquitous expression of MSTN in adult tissues. MSTN was detectable from zygote till hatching showed the maternal deposition of MSTN mRNA from oocytes and continuous expression during embryogenesis. Treatment of rare minnow with 1 mu g/L 17 alpha-methyl testosterone (MT) or 17 alpha-ethynyl estradiol (EE2) significantly impaired the body growth and increased the expression of MSTN mRNA detected by quantitative real time PCR. However, low dosage of MT (10 ng/L) had no significant effects on body growth and MSTN expression. The results suggest that the effects of exogenous sex steroids on growth can be mediated through MSTN. It is also suggested that low concentration of sex steroid (less than 10 ng/L) may have little effect on MSTN expression. (C) 2012 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	MESSENGER-RNA EXPRESSION ; ZEBRAFISH DANIO-RERIO ; RAINBOW-TROUT ; 17-ALPHA-ETHINYLESTRADIOL EE2 ; ONCORHYNCHUS-MYKISS ; BODY-COMPOSITION ; GENE-EXPRESSION ; SPARUS-AURATA ; MUSCLE MASS ; IGF-I
收录类别	SCI
资助信息	State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2005FB18]
语种	英语
WOS记录号	WOS:000305068800001
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16925]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yuhui,Peng, Kou,Liu, Xiaoyan,et al. Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus[J]. AQUACULTURE,2012,350:1-7.
APA	Yang, Yuhui.,Peng, Kou.,Liu, Xiaoyan.,Zhao, Dongmei.,Duan, Jundan.,...&Zhao, Haobin.(2012).Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus.AQUACULTURE,350,1-7.
MLA	Yang, Yuhui,et al."Effects of sex steroids on expression of myostatin in rare minnow, Gobiocypris rarus".AQUACULTURE 350(2012):1-7.