Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)

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	Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)
	Zhong, Chengrong 1,2; Song, Yanlong 1,2; Wang, Yaping 1; Li, Yongming 1; Liao, Lanjie 1; Xie, Shouqi 1; Zhu, Zuoyan 1; Hu, Wei 1
刊名	AQUACULTURE
	2012-08-01
卷号	356 页码:404-411
关键词	Growth hormone Transgenic common carp Homozygous line Biological characteristic Breed
ISSN号	0044-8486
通讯作者	Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	Higher productivity of cultivated fish is required to sustain the supply of fisheries products in the light of increasing demand. We established different lines of homozygous growth hormone (GH) transgenic common carp and analyzed their biological characteristics. Comparing transgenic and control larvae derived from the same control fish mother, we found there was no significant difference in fertilization and hatch rates, but that hatch timing was advanced and average total length was significantly longer in transgenic carp. Early growth performance and other biological characteristics were compared among homozygous, hemizygous and control fish. Both the #TG2 and #TG3 lines exhibited greater average body weight, specific growth rate (SGR), feed conversion efficiency (FCE) and protein retention efficiency (PRE), as well as lower feeding rate (FR) and lipid and energy content than control fish. The energy retention efficiency (ERE) was significantly lower in the #TG3 line, while no difference was observed between the #TG2 line and control fish. These results indicate that the GH transgene initiated effects on growth enhancement in the early developmental stages and played an important role in improving protein synthesis and lipolysis. Conversely, homozygous transgenic fish No. 14960 grew faster than the control fish, but the culture traits of its hemizygous offspring (#TG1 line) were comparatively reduced, including lower SGR, FCE, PRE and ERE levels, and higher FR and lipid contents. Our results suggest that even if fast-growing GH-transgenic homozygous parental fish are generated, we still need to practice classical selective breeding in order to produce transgenic fish with improved traits that can meet aquaculture demand. (C) 2012 Elsevier B. V. All rights reserved.
英文摘要	Higher productivity of cultivated fish is required to sustain the supply of fisheries products in the light of increasing demand. We established different lines of homozygous growth hormone (GH) transgenic common carp and analyzed their biological characteristics. Comparing transgenic and control larvae derived from the same control fish mother, we found there was no significant difference in fertilization and hatch rates, but that hatch timing was advanced and average total length was significantly longer in transgenic carp. Early growth performance and other biological characteristics were compared among homozygous, hemizygous and control fish. Both the #TG2 and #TG3 lines exhibited greater average body weight, specific growth rate (SGR), feed conversion efficiency (FCE) and protein retention efficiency (PRE), as well as lower feeding rate (FR) and lipid and energy content than control fish. The energy retention efficiency (ERE) was significantly lower in the #TG3 line, while no difference was observed between the #TG2 line and control fish. These results indicate that the GH transgene initiated effects on growth enhancement in the early developmental stages and played an important role in improving protein synthesis and lipolysis. Conversely, homozygous transgenic fish No. 14960 grew faster than the control fish, but the culture traits of its hemizygous offspring (#TG1 line) were comparatively reduced, including lower SGR, FCE, PRE and ERE levels, and higher FR and lipid contents. Our results suggest that even if fast-growing GH-transgenic homozygous parental fish are generated, we still need to practice classical selective breeding in order to produce transgenic fish with improved traits that can meet aquaculture demand. (C) 2012 Elsevier B. V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	SALMON ONCORHYNCHUS-KISUTCH ; TILAPIA OREOCHROMIS-NILOTICUS ; ANTIFREEZE PROTEIN GENE ; TIME QUANTITATIVE PCR ; COHO SALMON ; ATLANTIC SALMON ; BODY-COMPOSITION ; CTENOPHARYNGODON-IDELLUS ; ZYGOSITY DETERMINATION ; MISGURNUS-MIZOLEPIS
收录类别	SCI
资助信息	'863' High Technology Project[2011AA100404]; National Natural Science Foundation[30930069]; Chinese Academy of Sciences[KSCX2-EW-N-004]
语种	英语
WOS记录号	WOS:000306171100051
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16911]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Chengrong,Song, Yanlong,Wang, Yaping,et al. Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)[J]. AQUACULTURE,2012,356:404-411.
APA	Zhong, Chengrong.,Song, Yanlong.,Wang, Yaping.,Li, Yongming.,Liao, Lanjie.,...&Hu, Wei.(2012).Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.).AQUACULTURE,356,404-411.
MLA	Zhong, Chengrong,et al."Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.)".AQUACULTURE 356(2012):404-411.