Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata

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	Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata
	Jiang, Yongguang 1,2; Xiao, Peng 1,2; Yu, Gongliang 1; Sano, Tomoharu 3; Pan, Qianqian 1,2; Li, Renhui 1
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2012-04-01
卷号	78 期号:7 页码:2256-2263
ISSN号	0099-2240
通讯作者	Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
中文摘要	New insights into the distribution and biochemistry of the cyanotoxin cylindrospermopsin (CYN) have been provided by the recent determination of its biosynthesis gene cluster (cyr) in several cyanobacterial species. Raphidiopsis curvata CHAB1150 isolated from China was analyzed for CYN analogues. Only 7-deoxy-CYN was detected in the cell extracts. The cyr gene cluster of R. curvata CHAB1150 was sequenced, and the cyr genes of this strain were found to have extremely high similarities (96% to 100%) to those from other nostocalean species. These species include Cylindrospermopsis raciborskii AWT205, Aphanizomenon sp. strain 10E6, and Aphanizomenon ovalisporum ILC-146. Insertion mutation was identified within the cyrI gene, and transcripts of cyrI and another functional gene cyrJ were detected in R. curvata CHAB1150. General congruence between the phylogenetic trees based on both cyr and 16S rrn was displayed. Neutral evolution was found on the whole sequences of the cyr genes, and 0 to 89 negative selected codons were detected in each gene. Therefore, the function of CyrI is to catalyze the oxygenation of 7-deoxy-CYN in CYN biosynthesis. The transcripts of the mutated cyrI gene may result from polycistronic transcription. The high conservation of the cyr genes may be ascribed to purifying selection and horizontal gene transfer.
英文摘要	New insights into the distribution and biochemistry of the cyanotoxin cylindrospermopsin (CYN) have been provided by the recent determination of its biosynthesis gene cluster (cyr) in several cyanobacterial species. Raphidiopsis curvata CHAB1150 isolated from China was analyzed for CYN analogues. Only 7-deoxy-CYN was detected in the cell extracts. The cyr gene cluster of R. curvata CHAB1150 was sequenced, and the cyr genes of this strain were found to have extremely high similarities (96% to 100%) to those from other nostocalean species. These species include Cylindrospermopsis raciborskii AWT205, Aphanizomenon sp. strain 10E6, and Aphanizomenon ovalisporum ILC-146. Insertion mutation was identified within the cyrI gene, and transcripts of cyrI and another functional gene cyrJ were detected in R. curvata CHAB1150. General congruence between the phylogenetic trees based on both cyr and 16S rrn was displayed. Neutral evolution was found on the whole sequences of the cyr genes, and 0 to 89 negative selected codons were detected in each gene. Therefore, the function of CyrI is to catalyze the oxygenation of 7-deoxy-CYN in CYN biosynthesis. The transcripts of the mutated cyrI gene may result from polycistronic transcription. The high conservation of the cyr genes may be ascribed to purifying selection and horizontal gene transfer.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	2 GERMAN LAKES ; CYLINDROSPERMOPSIS-RACIBORSKII ; GENE-CLUSTER ; CYANOTOXINS CYLINDROSPERMOPSIN ; APHANIZOMENON-OVALISPORUM ; PROTEIN-SYNTHESIS ; UMEZAKIA-NATANS ; 1ST REPORT ; IDENTIFICATION ; STRAIN
收录类别	SCI
资助信息	State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501]
语种	英语
WOS记录号	WOS:000301344300021
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17057]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Natl Inst Environm Studies, Tsukuba, Ibaraki, Japan
推荐引用方式 GB/T 7714	Jiang, Yongguang,Xiao, Peng,Yu, Gongliang,et al. Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2012,78(7):2256-2263.
APA	Jiang, Yongguang,Xiao, Peng,Yu, Gongliang,Sano, Tomoharu,Pan, Qianqian,&Li, Renhui.(2012).Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,78(7),2256-2263.
MLA	Jiang, Yongguang,et al."Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 78.7(2012):2256-2263.