Recruiting alternative glucose utilization pathways for improving succinate production

	Recruiting alternative glucose utilization pathways for improving succinate production
	Tang, Jinlei 1,2,3; Zhu, Xinna 1,2; Lu, Jiao 1,2; Liu, Pingping 1,2; Xu, Hongtao 1,2; Tan, Zaigao 1,2; Zhang, Xueli 1,2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2013-03-01
卷号	97 期号:6 页码:2513-2520
关键词	Glucose utilization PTS Succinate Galactose permease Glucose facilitator Escherichia coli
英文摘要	The phosphoenolpyruvate (PEP): carbohydrate phosphotransferase system (PTS) of Escherichia coli was usually inactivated to increase PEP supply for succinate production. However, cell growth and glucose utilization rate decreased significantly with PTS inactivation. In this work, two glucose transport proteins and two glucokinases (Glk) from E. coli and Zymomonas mobilis were recruited in PTS- strains, and their impacts on glucose utilization and succinate production were compared. All PTS- strains recruiting Z. mobilis glucose facilitator Glf had higher glucose utilization rates than PTS- strains using E. coli galactose permease (GalP), which was suggested to be caused by higher glucose transport velocity and lower energetic cost of Glf. The highest rate obtained by combinatorial modulation of glf and glk (E. coli) (2.13 g/LaEuro cent h) was 81 % higher than the wild-type E. coli and 30 % higher than the highest rate obtained by combinatorial modulation of galP and glk (E. coli) . On the other hand, although glucokinase activities increased after replacing E. coli Glk with isoenzyme of Z. mobilis, glucose utilization rate decreased to 0.58 g/LaEuro cent h, which was assumed due to tight regulation of Z. mobilis Glk by energy status of the cells. For succinate production, using GalP led to a 20 % increase in succinate productivity, while recruiting Glf led to a 41 % increase. These efficient alternative glucose utilization pathways obtained in this work can also be used for production of many other PEP-derived chemicals, such as malate, fumarate, and aromatic compounds.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	RECOMBINANT ESCHERICHIA-COLI ; ZYMOMONAS-MOBILIS ; PYRUVATE-CARBOXYLASE ; ENGINEERED STRAINS ; TRANSPORT ; MUTANT ; GLUCOKINASE ; EXPRESSION ; YIELD ; SUGAR
收录类别	SCI
语种	英语
WOS记录号	WOS:000315650100021
公开日期	2012-08-29
内容类型	期刊论文
源URL	[http://localhost/handle/0/259]
专题	天津工业生物技术研究所_微生物代谢工程张学礼_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Tang, Jinlei,Zhu, Xinna,Lu, Jiao,et al. Recruiting alternative glucose utilization pathways for improving succinate production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2513-2520.
APA	Tang, Jinlei.,Zhu, Xinna.,Lu, Jiao.,Liu, Pingping.,Xu, Hongtao.,...&Zhang, Xueli.(2013).Recruiting alternative glucose utilization pathways for improving succinate production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2513-2520.
MLA	Tang, Jinlei,et al."Recruiting alternative glucose utilization pathways for improving succinate production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2513-2520.