Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production

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	Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production
	Zhu, Yueming; Men, Yan; Bai, Wei; Li, Xiaobo; Zhang, Lili; Sun, Yuanxia; Ma, Yanhe
刊名	BIOTECHNOLOGY LETTERS
	2012-10-01
卷号	34 期号:10 页码:1901-1906
关键词	Fructose D-Psicose D-Psicose 3-epimerase Ruminococcus Thermostability
英文摘要	The d-psicose 3-epimerase (DPE) gene from Ruminococcus sp. was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at pH 7.5-8.0 and 60 A degrees C. Activity was not dependent on the presence of metal ions; however, it became more thermostable with added Mn2+. The K (m) of the enzyme for d-psicose (48 mM) was lower than that for d-tagatose (230 mM), suggesting that d-psicose is the optimum substrate. More importantly, the thermostability of the novel DPE from Ruminococcus is the strongest among all of the d-psicose and d-tagatose 3-epimerases and may be suitable for the industrial production of d-psicose from fructose.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	D-TAGATOSE 3-EPIMERASE ; PSEUDOMONAS CICHORII ST-24 ; D-FRUCTOSE ; AGROBACTERIUM-TUMEFACIENS ; CLONING ; ENZYME
收录类别	SCI
语种	英语
WOS记录号	WOS:000310429800019
公开日期	2012-08-29
内容类型	期刊论文
源URL	[http://localhost/handle/0/260]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Yueming,Men, Yan,Bai, Wei,et al. Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production[J]. BIOTECHNOLOGY LETTERS,2012,34(10):1901-1906.
APA	Zhu, Yueming.,Men, Yan.,Bai, Wei.,Li, Xiaobo.,Zhang, Lili.,...&Ma, Yanhe.(2012).Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production.BIOTECHNOLOGY LETTERS,34(10),1901-1906.
MLA	Zhu, Yueming,et al."Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production".BIOTECHNOLOGY LETTERS 34.10(2012):1901-1906.