Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei
	Xue, Chun-Ying2 ; Xue, Heng-Gang 1
刊名	PLANTA MEDICA
	2008-09-01
卷号	74 期号:11 页码:1416-1420
关键词	traditional Chinese medicinal plants Drynaria fortunei Polypodiaceae real-time scorpion polymerase chain reaction
ISSN号	0032-0943
通讯作者	xuegangdyx@yahoo.com.cn
英文摘要	DNA sequence analysis of the trnL-trnF spacer region and real-time scorpion polymerase chain reaction were exploited for their applications in the differentiation and quantification of the traditional Chinese medicinal plants Drynaria fortunei from five related adulterants: D. mollis, D. quercifolia, D. rigidula, D. sparsisora, and Pseudodrynaria coronans. The data demonstrated that variations in the trnL-trnF spacer regions were very low at the intraspecific level but extremely high at the interspecific level, meaning that they could be easily distinguished at the DNA level. The sequence difference allowed effective and reliable differentiation of D. fortunei from adulterants by real-time scorpion PCR. Furthermore, a quantification methodology was carried out to quantify D. fortunei.
学科主题	Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy
类目[WOS]	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]	Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]	MOLECULAR AUTHENTICATION
收录类别	SCI
资助信息	Natural Science Foundation of China (NSFC) [30770153]; Natural Science Foundation of Yunnan (NSFN) [2006C 0050 M]
语种	英语
WOS记录号	WOS:000260174300018
公开日期	2012-07-18
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/13955]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Xue, Chun-Ying,Xue, Heng-Gang. Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei[J]. PLANTA MEDICA,2008,74(11):1416-1420.
APA	Xue, Chun-Ying,&Xue, Heng-Gang.(2008).Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei.PLANTA MEDICA,74(11),1416-1420.
MLA	Xue, Chun-Ying,et al."Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei".PLANTA MEDICA 74.11(2008):1416-1420.