Assembling of bioorthogonal redox systems

	Assembling of bioorthogonal redox systems
	Ji DB(纪德彬) ; Wang L(王磊) ; Liu WJ(刘武军) ; Zhao ZB(赵宗保)
	2011
会议名称	2011 cold spring harbor asia symposium
会议日期	2011-11-7
会议地点	苏州
页码	61-0
通讯作者	赵宗保
中文摘要	biological redox chemistry is catalyzed by numerous enzymes that depend on a few cofactors, including the omnipresent cofactor, nicotinamide adenine dinucleotide (nad). nad is also involved in various non-redox processes. it remains challenging to disconnect one particular nad-dependent reaction from all others. here we present a bioorthogonal systen that catalyzes the oxidative decarboxylation of l-malate with a dedicated abiotic cofactor, nicotinamide flucytosine dinucleotide (nfcd). by screening the multi-site saturated mutagenesis libraries of the nad-dependent malic enzyme (me), we identified the mutant me-l310r/q401c, which showed excellent activity with nfcd, yet marginal activity with nad. inspired by these observations, we mutated the d-lactate dehydrogenase (dldh) to dldh-v152r, which was fully active with nfcd. when coupled with dldh-v 152r, me-l310r/q401c required only a catalytic amount of nfcd to convert l-malate. our results opened the window to engineer bioorthogonal redox systems for a wide variety of applications in systems biology and synthetic biology.
合作状况	墙报
会议主办者	冷泉港实验室
会议录	abstracts of papers presented at the 2011 cold spring harbor asia symposium
会议录出版者	待补充
会议录出版地	待补充
学科主题	生物化学
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/116041]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Ji DB,Wang L,Liu WJ,et al. Assembling of bioorthogonal redox systems[C]. 见:2011 cold spring harbor asia symposium. 苏州. 2011-11-7.