Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis

	Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
	Zhang, Biaoming 1,2; Cao, Xupeng 1; Cheng, Xiaofei 4; Wu, Peichun 1; Xiao, Tonghu 4; Zhang, Wei 1,3
刊名	biotechnology letters
	2011-03-01
卷号	33 期号:3 页码:545-548
关键词	Corallina officinalis Heat treatment Protein purification Vanadium-dependent bromoperoxidase
ISSN号	待补充
通讯作者	weizhang
产权排序	1,1
中文摘要	efficient purification with high recovery of vanadium bromoperoxidase from corallina officinalis
英文摘要	a novel, simple and highly efficient process for purifying vanadium bromoperoxidase from corallina officinalis is reported. the key innovation is adding 0.5 mm sodium orthovanadate to the crude cell extract followed by heating at 70a degrees c for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. combining the heat treatment with ammonium sulfate precipitation and deae-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. finally, a specific activity of 310 u/mg, a 27-fold purification of the crude enzyme solution was produced.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	pilulifera
收录类别	SCI
语种	英语
WOS记录号	WOS:000287502500014
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115393]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia 4.Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,et al. Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis[J]. biotechnology letters,2011,33(3):545-548.
APA	Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,Wu, Peichun,Xiao, Tonghu,&Zhang, Wei.(2011).Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis.biotechnology letters,33(3),545-548.
MLA	Zhang, Biaoming,et al."Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis".biotechnology letters 33.3(2011):545-548.