The development of an evaluation method for capture columns used in two-dimensional liquid chromatography

	The development of an evaluation method for capture columns used in two-dimensional liquid chromatography
	Cao, Liwei 1,2; Yu, Danhua 1; Wang, Xinliang 1; Ke, Yanxiong 1; Jin, Yu 1; Liang, Xinmiao 1,2
刊名	analytica chimica acta
	2011-11-07
卷号	706 期号:1 页码:184-190
关键词	Capture column Evaluation method Two-dimensional liquid chromatography Separation materials
ISSN号	待补充
通讯作者	金郁 ; 梁鑫淼
产权排序	2,1
中文摘要	the development of an evaluation method for capture columns used in two-dimensional liquid chromatography
英文摘要	capture columns are important interface tools for on line two-dimensional liquid chromatography (2d-lc). in this study, a systematic method was developed to evaluate and optimize the capture ability of capture columns by off-line method. first, the parameter delta t(r) (delta t(r) = t(2)-t(1)-t(0)-w) was introduced to quantitatively represent the capture ability of the capture column by connecting a capture column behind the first dimensional column. based on the value of delta t(r), an appropriate capture column was selected after the first dimensional column was fixed. then, the capture ability of the selected column was promoted by adjusting the mobile phase of the first dimensional column. capture ability was also optimized using complex sample analysis software system (csass) software. second, the elution mode of the trapped compounds on the capture column was investigated by connecting the capture column before the second dimensional column. more specifically, in mode i. capture column was connected to the second dimension without changing the flow rate direction and the trapped compounds must pass through the capture column and be eluted into the second dimensional column. the contrary connection mode was mode ii. it was found that mode i is more suitable method for 2d-lc. finally, an off-line reversed-phase/hydrophilic interaction liquid chromatography two-dimensional liquid chromatography (rp/hilic 2d-lc) system with a c18 capture column was developed to demonstrate the practical application of this method. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	reversed-phase ; mass-spectrometry ; performance ; separation ; system ; protein ; fractionation ; optimization ; pressure ; samples
收录类别	SCI
语种	英语
WOS记录号	WOS:000296755300020
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115349]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Cao, Liwei,Yu, Danhua,Wang, Xinliang,et al. The development of an evaluation method for capture columns used in two-dimensional liquid chromatography[J]. analytica chimica acta,2011,706(1):184-190.
APA	Cao, Liwei,Yu, Danhua,Wang, Xinliang,Ke, Yanxiong,Jin, Yu,&Liang, Xinmiao.(2011).The development of an evaluation method for capture columns used in two-dimensional liquid chromatography.analytica chimica acta,706(1),184-190.
MLA	Cao, Liwei,et al."The development of an evaluation method for capture columns used in two-dimensional liquid chromatography".analytica chimica acta 706.1(2011):184-190.