A new method for chemical identification based on orthogonal parallel liquid chromatography separation and accurate molecular weight confirmation

	A new method for chemical identification based on orthogonal parallel liquid chromatography separation and accurate molecular weight confirmation
	Zeng, Jing; Zhang, Xiuli; Guo, Zhimou; Feng, Jiatao; Xue, Xingya; Liang, Xinmiao
刊名	journal of chromatography a
	2011-04-01
卷号	1218 期号:13 页码:1749-1755
关键词	Orthogonal parallel separation Retention time Accurate molecular weight Chemical identification Flavonoids Library searching
ISSN号	待补充
通讯作者	薛兴亚 ; 梁鑫淼
产权排序	1,1
中文摘要	a new method for chemical identification based on orthogonal parallel liquid chromatography separation and accurate molecular weight confirmation
英文摘要	recent advances in the theory and application of orthogonal lc separation have allowed for the establishment of a more effective method for the chemical identification of target compounds in complex samples, especially structurally similar compounds. in this study, a new chemical identification method based on orthogonal parallel separation and accurate molecular weight confirmation was developed. an orthogonal separation system consisting of an xterra ms c-18 column, a home-made click oeg column, and a click cd column was established for separation and identification. in addition, 82 flavonoids were selected as references, to be used for the construction of a library. retention times of each reference flavonoid on each column and accurate molecular weights were recorded and imported into a searchable library as "tags" for the unknown screening. for the method validation, two complex mixtures, fractions of dalbergia odorifera t. chen and scutellaria baicalensis georgi, specifically, were separated and identified. in total, nine compounds were unequivocally identified by retention time and confirmation of accurate molecular weight, demonstrating that this method is suitable and efficient for the chemical identification of complex samples. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	inter-laboratory transferability ; tandem mass-spectrometry ; oligo(ethylene glycol) ; rapid identification ; qualitative-analysis ; stationary-phase ; spectral library ; system ; column ; drugs
收录类别	SCI
语种	英语
WOS记录号	WOS:000288837400008
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115344]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Lab Nat Med, Dalian 116023, Liaoning Prov, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Jing,Zhang, Xiuli,Guo, Zhimou,et al. A new method for chemical identification based on orthogonal parallel liquid chromatography separation and accurate molecular weight confirmation[J]. journal of chromatography a,2011,1218(13):1749-1755.
APA	Zeng, Jing,Zhang, Xiuli,Guo, Zhimou,Feng, Jiatao,Xue, Xingya,&Liang, Xinmiao.(2011).A new method for chemical identification based on orthogonal parallel liquid chromatography separation and accurate molecular weight confirmation.journal of chromatography a,1218(13),1749-1755.
MLA	Zeng, Jing,et al."A new method for chemical identification based on orthogonal parallel liquid chromatography separation and accurate molecular weight confirmation".journal of chromatography a 1218.13(2011):1749-1755.