Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity

	Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity
	Zhang, Liyuan 1,2; Xu, Jin 3; Sun, Liangliang 1,2; Ma, Junfeng 1,2; Yang, Kaiguang 1; Liang, Zhen 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	analytical and bioanalytical chemistry
	2011-04-01
卷号	399 期号:10 页码:3399-3405
关键词	ZrO(2) aerogel Phosphopeptide Enrichment Mass spectrometry
ISSN号	待补充
通讯作者	张丽华
产权排序	1,1
中文摘要	zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity
英文摘要	in this study, zirconium oxide (zro(2)) aerogel was synthesized via a green sol-gel approach, with zirconium oxychloride, instead of the commonly used alkoxide with high toxicity, as the precursor. with such material, phosphopeptides from the digests of 4 pmol of beta-casein with the coexistence of 100 times (mol ratio) bsa could be selectively captured, and identified by maldi-tof ms. due to the large surface area (416.0 m(2) g(-1)) and the mesoporous structure (the average pore size of 10.2 nm) of zro(2) aerogel, a 20-fold higher loading capacity for phosphopeptide, ykvpqleivpn[ps]aeer (mw 1952.12), was obtained compared to that of commercial zro(2) microspheres (341.5 vs. 17.87 mg g(-1)). the metal oxide aerogel was further applied in the enrichment of phosphopeptides from 100 ng nonfat milk, and 17 phosphopeptides were positively identified, with a 1.5-fold improvement in phosphopeptide detection compared with previously reported results. these results demonstrate that zro(2) aerogel can be a powerful enrichment material for phosphoproteome study.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	ion affinity-chromatography ; tin dioxide microspheres ; phosphorylated peptides ; mass-spectrometry ; phosphoproteome analysis ; nanoparticles ; performance ; adsorbent ; growth ; carbon
收录类别	SCI
语种	英语
WOS记录号	WOS:000288398700009
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115312]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.China Med Univ, Shengjing Hosp, Dept Hepat Biliary Pancreas & Transplantat Surg, Shenyang 110004, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Liyuan,Xu, Jin,Sun, Liangliang,et al. Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity[J]. analytical and bioanalytical chemistry,2011,399(10):3399-3405.
APA	Zhang, Liyuan.,Xu, Jin.,Sun, Liangliang.,Ma, Junfeng.,Yang, Kaiguang.,...&Zhang, Yukui.(2011).Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity.analytical and bioanalytical chemistry,399(10),3399-3405.
MLA	Zhang, Liyuan,et al."Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity".analytical and bioanalytical chemistry 399.10(2011):3399-3405.