Proteomics of desiccation tolerance during development and germination of maize embryos | |
Huang, Hui1,2; Moller, Ian Max3; Song, Song-Quan1 | |
刊名 | JOURNAL OF PROTEOMICS |
2012-02-02 | |
卷号 | 75期号:4页码:1247-1262 |
关键词 | Desiccation tolerance Development Germination Maize embryos Proteomic analysis |
ISSN号 | 1874-3919 ; 1874-3919 |
通讯作者 | Song, SQ (reprint author), Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China, sqsong@ibcas.ac.cn ; Song, SQ (reprint author), Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China, sqsong@ibcas.ac.cn |
英文摘要 | Maize seeds were used to identify the key embryo proteins involved in desiccation tolerance during development and germination. Immature maize embryos (28N) during development and mature embryos imbibed for 72 h (72HN) are desiccation sensitive. Mature maize embryos (52N) during development are desiccation tolerant Thiobarbituric acid reactive substance and hydrogen peroxide contents decreased and increased with acquisition and loss of desiccation tolerance, respectively. A total of 111 protein spots changed significantly (1.5 fold increase/decrease) in desiccation-tolerant and -sensitive embryos before (28N, 52N and 72HN) and after (280, 520 and 72HD) dehydration. Nine pre-dominantly proteins, 17.4 kDa Class I heat shock protein 3, late embiyogenesis abundant protein EMB564, outer membrane protein, globulin 2, TPA:putative cystatin, NBS-LFtR resistance-like protein RGC456, stress responsive protein, major allergen Bet v 1.01C and proteasome subunit alpha type 1, accumulated during embryo maturation, decreased during germination and increased in desiccation-tolerant embryos during desiccation. Two proteins, Rhd6-like 2 and low-molecular-weight heat shock protein precursor, showed the inverse pattern. We infer that these eleven proteins are involved in seed desiccation tolerance. We conclude that desiccation-tolerant embryos make more economical use of their resources to accumulate protective molecules and antioxidant systems to deal with maturation drying and desiccation treatment. (C) 2011 Elsevier B.V. All rights reserved. |
学科主题 | Biochemical Research Methods ; Biochemical Research Methods |
类目[WOS] | Biochemical Research Methods |
研究领域[WOS] | Biochemistry & Molecular Biology |
关键词[WOS] | EMBRYOGENESIS-ABUNDANT PROTEIN ; HEAT-SHOCK PROTEINS ; RICE ORYZA-SATIVA ; ARABIDOPSIS-THALIANA ; HYDROGEN-PEROXIDE ; SEED-GERMINATION ; ACTIVE OXYGEN ; BEAN-SEEDS ; STRESS ; PLANTS |
收录类别 | SCI |
资助信息 | National Natural Sciences Foundation of China[30870223]; Chinese Academy of Sciences[KZCX2-YW-414]; National Natural Sciences Foundation of China[30870223]; Chinese Academy of Sciences[KZCX2-YW-414] |
语种 | 英语 |
WOS记录号 | WOS:000300533600015 |
公开日期 | 2012-06-07 ; 2012-06-07 |
内容类型 | 期刊论文 |
源URL | [http://ir.kib.ac.cn/handle/151853/10527] |
专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 3.Aarhus Univ, Dept Mol Biol & Biotechnol, DK-4200 Slagelse, Denmark |
推荐引用方式 GB/T 7714 | Huang, Hui,Moller, Ian Max,Song, Song-Quan. Proteomics of desiccation tolerance during development and germination of maize embryos[J]. JOURNAL OF PROTEOMICS,2012,75(4):1247-1262. |
APA | Huang, Hui,Moller, Ian Max,&Song, Song-Quan.(2012).Proteomics of desiccation tolerance during development and germination of maize embryos.JOURNAL OF PROTEOMICS,75(4),1247-1262. |
MLA | Huang, Hui,et al."Proteomics of desiccation tolerance during development and germination of maize embryos".JOURNAL OF PROTEOMICS 75.4(2012):1247-1262. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论