恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率

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	恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率
	王辉林 ; 李江华 ; 刘龙 ; 宋江宁 ; 堵国成
刊名	生物工程学报
	2012-08-25
卷号	28 期号:8 页码:937~949
中文摘要	为了提高重组毕赤酵母生产碱性果胶酶 (Alkaline polygalacturonate lyase，PGL) 的比速率，开发了一种新的恒细胞密度发酵策略。通过不同的甲醇流加方式，实现发酵过程细胞密度的合理控制。实验结果表明：控制细胞密度为75 g/L的策略为最优，最终单位发酵液体积生产强度和单位菌体生产强度为6.11 U/(mL·h) 和81.5 U/(g·h)，分别比传统高密度发酵提高了42.1％和191.2％，最终PGL酶活为441.9 U/mL。此外，该策略还具有提高细胞活性和降低蛋白酶降解作用
英文摘要	In order to enhance the alkaline polygalacturonate lyase (PGL) productivity by Pichia pastoris, we developed a constant cell concentration culture strategy by methanol feeding (called as CCCM culture) used in the continuous cultures. We controlled reasonable cell concentrations in the bioprocess by different strategies of methanol feeding. Using this CCCM culture with DCW 75 g/L, we significantly enhanced the PGL productivity (QV) and the average specific enzyme production rate (QX) of PGL to 6.11 U/(mL·h) and 81.5 U/(g·h), increased by 42.1% and 191.2% than the fed-batch culture with high cell density, respectively. The final PGL activity was 441.9 U/mL. Moreover, the extracellular protease concentration is 1.9 mg/L and the cell viability is more than 94% after 120 hour induction. The results show that this new strategy is advantageous in reducing proteolytic degradation and enhancing cell viability.
公开日期	2012-06-01
内容类型	期刊论文
源URL	[http://localhost/handle/0/250]
专题	天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室宋江宁_期刊论文
推荐引用方式 GB/T 7714	王辉林,李江华,刘龙,等. 恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率[J]. 生物工程学报,2012,28(8):937~949.
APA	王辉林,李江华,刘龙,宋江宁,&堵国成.(2012).恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率.生物工程学报,28(8),937~949.
MLA	王辉林,et al."恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率".生物工程学报 28.8(2012):937~949.