Crystal structures of D-psicose 3-epimerase from Clostridum cellulolyticum H10 and its complex with ketohexose sugars

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	Crystal structures of D-psicose 3-epimerase from Clostridum cellulolyticum H10 and its complex with ketohexose sugars
	Hsiu-Chien Chan ; Yueming Zhu ; Yumei Hu ; Tzu-Ping Ko ; Chun-Hsiang Huang ; Feifei Ren ; Chun-Chi Chen ; Yanhe Ma ; Rey-Ting Guo ; Yuanxia Sun
刊名	Protein&Cell
	2012-03
卷号	3 期号:2 页码:123-131
英文摘要	D-psicose 3-epimerase (DPEase) is demonstrated to be useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature. Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase. In this study, the crystal structure of the C. cellulolyticum DPEase was determined. The enzyme assembles into a tetramer and each subunit shows a (β/α)(8) TIM barrel fold with a Mn(2+) metal ion in the active site. Additional crystal structures of the enzyme in complex with substrates/products (D-psicose, D-fructose, D-tagatose and D-sorbose) were also determined. From the complex structures of C. cellulolyticum DPEase with D-psicose and D-fructose, the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues. Accordingly, based on these ketohexose-bound complex structures, a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here. These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis.
公开日期	2012-06-01
内容类型	期刊论文
源URL	[http://localhost/handle/0/243]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
推荐引用方式 GB/T 7714	Hsiu-Chien Chan,Yueming Zhu,Yumei Hu,et al. Crystal structures of D-psicose 3-epimerase from Clostridum cellulolyticum H10 and its complex with ketohexose sugars[J]. Protein&Cell,2012,3(2):123-131.
APA	Hsiu-Chien Chan.,Yueming Zhu.,Yumei Hu.,Tzu-Ping Ko.,Chun-Hsiang Huang.,...&Yuanxia Sun.(2012).Crystal structures of D-psicose 3-epimerase from Clostridum cellulolyticum H10 and its complex with ketohexose sugars.Protein&Cell,3(2),123-131.
MLA	Hsiu-Chien Chan,et al."Crystal structures of D-psicose 3-epimerase from Clostridum cellulolyticum H10 and its complex with ketohexose sugars".Protein&Cell 3.2(2012):123-131.