Single cell Raman spectroscopy for cell sorting and imaging | |
Li, Mengqiu1; Xu, Jian2,3![]() | |
刊名 | CURRENT OPINION IN BIOTECHNOLOGY
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2012-02-01 | |
卷号 | 23期号:1页码:56-63 |
中文摘要 | Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular ‘fingerprint’ of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SRS) and surface enhanced Raman scattering (SERS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping. |
英文摘要 | Single cell Raman spectroscopy (SCRS) is a non-invasive and label-free technology, allowing in vivo and multiple parameter analysis of individual living cells. A single cell Raman spectrum usually contains more than 1000 Raman bands which provide rich and intrinsic information of the cell (e.g. nucleic acids, protein, carbohydrates and lipids), reflecting cellular genotypes, phenotypes and physiological states. A Raman spectrum serves as a molecular 'fingerprint' of a single cell, making it possible to differentiate various cells including bacterial, protistan and animal cells without prior knowledge of the cells. However, a key drawback of SCRS is the fact that spontaneous Raman signals are naturally weak; this review discusses recent research progress in significantly enhancing and improving the signal of spontaneous Raman spectroscopy, including resonance Raman spectroscopy (RRS), coherent anti-Stokes Raman spectroscopy (CARS), stimulated Raman spectroscopy (SIRS) and surface enhanced Raman scattering (SEAS). This review focuses on the biotechnological development and the associated applications of SCRS, including Raman activated cell sorting (RACS) and Raman imaging and mapping. |
学科主题 | 功能基因组 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biochemical Research Methods ; Biotechnology & Applied Microbiology |
研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
关键词[WOS] | IN-SITU HYBRIDIZATION ; FLUORESCENCE MICROSCOPY ; BACTERIAL-CELLS ; MICROBIAL-CELLS ; BLOOD-CELLS ; SCATTERING ; MICROSPECTROSCOPY ; COHERENT ; MOLECULES ; CYTOMETRY |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000300868100010 |
公开日期 | 2012-06-01 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/962] ![]() |
专题 | 青岛生物能源与过程研究所_单细胞中心 |
作者单位 | 1.Univ Sheffield, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,et al. Single cell Raman spectroscopy for cell sorting and imaging[J]. CURRENT OPINION IN BIOTECHNOLOGY,2012,23(1):56-63. |
APA | Li, Mengqiu,Xu, Jian,Romero-Gonzalez, Maria,Banwart, Steve A.,&Huang, Wei E..(2012).Single cell Raman spectroscopy for cell sorting and imaging.CURRENT OPINION IN BIOTECHNOLOGY,23(1),56-63. |
MLA | Li, Mengqiu,et al."Single cell Raman spectroscopy for cell sorting and imaging".CURRENT OPINION IN BIOTECHNOLOGY 23.1(2012):56-63. |
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