Versatile synthesis of temperature-sensitive polypeptides by click grafting of oligo(ethylene glycol)

	Versatile synthesis of temperature-sensitive polypeptides by click grafting of oligo(ethylene glycol)
	Cheng YL ; He CL ; Xiao CS ; Ding JX ; Zhuang XL ; Chen XS
刊名	polymer chemistry
	2011
卷号	2 期号:11 页码:2627-2634
关键词	TRANSFER RADICAL POLYMERIZATION RING-OPENING POLYMERIZATION BLOCK-COPOLYMER HYDROGELS DRUG-DELIVERY 2-(2-METHOXYETHOXY)ETHYL METHACRYLATE POLY(L-GLUTAMIC ACID) RESPONSIVE POLYMERS DIBLOCK COPOLYMERS CIRCULAR-DICHROISM AQUEOUS-SOLUTIONS
ISSN号	1759-9954
通讯作者	chen xs
中文摘要	a series of novel temperature-sensitive polypeptides were synthesized by ring opening polymerization (rop) of gamma-propargyl-l-glutamate n-carboxyanhydride (plg-nca) and subsequent click reaction between the pendant alkyne groups and 1-(2-methoxyethoxy)-2-azidoethane (meo(2)-n(3)) or 1-(2-(2-methoxyethoxy) ethoxy)-2-azidoethane (meo(3)-n(3)). the efficient click grafting and structure of the resultant copolymers were verified by (1)h nmr, (13)c nmr and gpc. all the copolymers hold alpha-helix conformation, and could self-assemble into amphiphilic nanoparticles in aqueous solution with hydrodynamic radii (r(h)) of 32.3-62.8 nm. the graft copolymers exhibited sharp temperature-dependent phase transitions, and the lcst could be adjusted from 22.3 to 74.1 degrees c by varying the molecular weight, the length of the oeg side chain, the polymer concentration and salt concentration. mtt assays revealed that the graft copolymers exhibited no detectable cytotoxicity at all test concentrations up to 1 mg ml(-1). in vitro degradation tests demonstrated that the graft copolymers could be degraded by proteinase k. the drug release behaviors from the pplg(112)-g-meo(2) nanoparticles were evaluated at 37 degrees c and 15 degrees c using doxorubicin (dox) as a model drug. the drug release behavior displayed thermosensitivity, and a sustained release profile was observed at physiological temperature. these results suggested that the novel biodegradable and biocompatible polypeptide derivatives with adjustable temperature sensitivity could be a promising material for biomedical applications.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000295811400024
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44904]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Cheng YL,He CL,Xiao CS,et al. Versatile synthesis of temperature-sensitive polypeptides by click grafting of oligo(ethylene glycol)[J]. polymer chemistry,2011,2(11):2627-2634.
APA	Cheng YL,He CL,Xiao CS,Ding JX,Zhuang XL,&Chen XS.(2011).Versatile synthesis of temperature-sensitive polypeptides by click grafting of oligo(ethylene glycol).polymer chemistry,2(11),2627-2634.
MLA	Cheng YL,et al."Versatile synthesis of temperature-sensitive polypeptides by click grafting of oligo(ethylene glycol)".polymer chemistry 2.11(2011):2627-2634.