Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy

	Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy
	Shi Y ; Guo CL ; Sun YJ ; Liu ZL ; Xu FG ; Zhang Y ; Wen ZW ; Li ZA
刊名	biomacromolecules
	2011
卷号	12 期号:3 页码:797-803
关键词	BOVINE SERUM-ALBUMIN HERRING SPERM DNA CYANOBACTERIAL TOXINS LIQUID-CHROMATOGRAPHY SPECTROSCOPY DAMAGE PROTEINS BINDING SPECTROMETRY INHIBITION
ISSN号	1525-7797
通讯作者	li za
中文摘要	microcystin-lr (mc-lr) is one of the hepatotoxins produced by cyanobacteria in the eutrophicated fresh water. in this work, the minor groove binding mode of mc-lr to plasmid dna was explored by using uv and fluorescence spectra, and the binding characteristics of mc-lr for plasmid dna were calculated via the fluorescence quenching of ethidium bromide (eb) and mole ratio method. furthermore, atomic force microscopy (afm) was used to observe dna morphology change in the presence of mc-lr. with the increasing concentration of, mc-lr, circle dna strands twined gradually to rod condensates. the possible reason for the condensation might be the masking of the electrostatic repulsion between dna double strands by mc-lr the present study might provide useful information for the pathopoiesis mechanism of mc-lr. more, because the condensation of dna could affect the progresses of gene expression and protein transcription, it may implicate another trend to explore the nosogenesis of mc-lr.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000288183100034
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44787]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Shi Y,Guo CL,Sun YJ,et al. Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy[J]. biomacromolecules,2011,12(3):797-803.
APA	Shi Y.,Guo CL.,Sun YJ.,Liu ZL.,Xu FG.,...&Li ZA.(2011).Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy.biomacromolecules,12(3),797-803.
MLA	Shi Y,et al."Interaction between DNA and Microcystin-LR Studied by Spectra Analysis and Atomic Force Microscopy".biomacromolecules 12.3(2011):797-803.