A sensitive, rapid and inexpensive way to assay pesticide toxicity based on electrochemical biosensor

	A sensitive, rapid and inexpensive way to assay pesticide toxicity based on electrochemical biosensor
	Yong DM ; Liu C ; Yu DB ; Dong SJ
刊名	talanta
	2011
卷号	84 期号:1 页码:7-12
关键词	BIOCHEMICAL OXYGEN-DEMAND ATOMIC-FORCE MICROSCOPY ESCHERICHIA-COLI FERRICYANIDE MICROORGANISMS MECHANISM MICREDOX BOD
ISSN号	0039-9140
通讯作者	dong sj
中文摘要	we reported a rapid toxicity assay method using electrochemical biosensor for pesticides, escherichia coli (e. coli) was taken as a model microorganism for test. in this method, we adopted ferricyanide instead of natural electron acceptor o(2), and then microbial oxidation was substantially accelerated. toxicity assays measured the effect of toxic materials on the metabolic activity of microorganisms. the current signal of ferrocyanide produced from the metabolism was proven to be directly related to the toxicity, which could be amplified by ultramicroelectrode array (umea). the ratio of the electrochemical signals, recorded in the presence and absence of toxin, provided an index of inhibition. accordingly, a direct toxicity assessment (dta) based on chronoamperometry was proposed to detect the effect of toxic chemicals on microorganisms. 3,5-dichlorophenol (dcp) was taken as the reference toxicant, its ic50 was estimated to be 8.0 mg/l. three pesticides were examined using this method. ic50 values of 6.5 mg/l for ametryn, 22 mg/l for fenamiphos and 5.7 mg/l for endosulfan were determined and in line with ec50 values reported in the literature. atomic force microscopy (afm) was also used for morphology characterization of e. coli induced by three pesticides. these results confirmed the present electrochemical method used is reliable. in addition, the electrochemical method is a sensitive, rapid and inexpensive way for toxicity assays of pesticides. (c) 2011 published by elsevier b.v.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000288342400002
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44762]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Yong DM,Liu C,Yu DB,et al. A sensitive, rapid and inexpensive way to assay pesticide toxicity based on electrochemical biosensor[J]. talanta,2011,84(1):7-12.
APA	Yong DM,Liu C,Yu DB,&Dong SJ.(2011).A sensitive, rapid and inexpensive way to assay pesticide toxicity based on electrochemical biosensor.talanta,84(1),7-12.
MLA	Yong DM,et al."A sensitive, rapid and inexpensive way to assay pesticide toxicity based on electrochemical biosensor".talanta 84.1(2011):7-12.