High-affinity peptide against MT1-MMP for in vivo tumor imaging

	High-affinity peptide against MT1-MMP for in vivo tumor imaging
	Zhu L ; Wang HL ; Wang L ; Wang Y ; Jiang K ; Li C ; Ma QJ ; Gao S ; Wang LP ; Li W ; Cai MJ ; Wang HD ; Niu G ; Lee S ; Yang W ; Fang XX ; Chen XY
刊名	journal of controlled release
	2011
卷号	150 期号:3 页码:248-255
关键词	TYPE-1 MATRIX-METALLOPROTEINASE INTEGRIN ALPHA(V)BETA(3) EXPRESSION 1-MATRIX METALLOPROTEINASE PERICELLULAR PROTEOLYSIS CELL MIGRATION PHAGE DISPLAY MEMBRANE INVASION CANCER IDENTIFICATION
ISSN号	0168-3659
通讯作者	yang w
中文摘要	membrane type-1 matrix metalloproteinase (mt1-mmp) is a key member of the matrix metalloproteinase (mmp) family. it participates in pericellular proteolysis of extracellular matrix (ecm) macromolecules and is essential for many biological and pathological processes, such as tumor development, angiogenesis and metastasis. a ligand that specifically binds to mt1-mmp may facilitate the labeling of this molecule, allow imaging at the cellular and organism levels, and provide a means for targeted drug delivery specific to mt1-mmp. a non-substrate mt1-mmp binding peptide was identified by screening a ph.d.-12 (tm) phage display peptide library and conjugated with near-infrared fluorescent (nirf) dye cy5.5 for tumor imaging. peptide hwkhlhntktfl (denoted as mt1-af7p) showed high mt1-mmp binding affinity. computer modeling verified that mt1-af7p binds to the mt-loop region of mt1-mmp and interacts with mt1-mmp through hydrogen bonding and hydrophobic interactions. mda-mb-435 xenografts with high mt1-mmp expression had significantly higher tumor accumulation and better tumor contrast than the low mt1-mmp expressing a549 xenografts after intravenous injection of cy5.5-mt1-af7p. using nirf imaging, we have demonstrated specific targeting of mt1-af7p to mt1-mmp-expressing tumors. thus, mt1-af7p is an important tool for noninvasive monitoring of mt1-mmp expression in tumors, and it shows great potential as an imaging agent for mt1-mmp-positive tumors. published by elsevier b.v.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000291131500003
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44748]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhu L,Wang HL,Wang L,et al. High-affinity peptide against MT1-MMP for in vivo tumor imaging[J]. journal of controlled release,2011,150(3):248-255.
APA	Zhu L.,Wang HL.,Wang L.,Wang Y.,Jiang K.,...&Chen XY.(2011).High-affinity peptide against MT1-MMP for in vivo tumor imaging.journal of controlled release,150(3),248-255.
MLA	Zhu L,et al."High-affinity peptide against MT1-MMP for in vivo tumor imaging".journal of controlled release 150.3(2011):248-255.