Screening kinase inhibitors with microarray-based Raman spectroscopic assay

	Screening kinase inhibitors with microarray-based Raman spectroscopic assay
	Li T ; Liu DJ ; Wang ZX
刊名	analytical methods
	2011
卷号	3 期号:4 页码:1003-1007
关键词	GOLD NANOPARTICLE PROBES PROTEIN-KINASES DESIGN PHOSPHORYLATION SPECIFICITY DISCOVERY LIBRARY ARRAYS POTENT SITE
ISSN号	1759-9660
通讯作者	wang zx
中文摘要	in this paper, a microarray-based surface-enhanced raman spectroscopic (sers) assay for screening kinase inhibitors was demonstrated by the interactions of two kinases, pka (the alpha-catalytic subunit of cyclic adenosine 5'-monophosphate (camp) dependent protein kinase a) and lck (leukocyte-specific protein tyrosine kinase) with 80 potent kinase inhibitors from a commercial inhibitor library. in this assay, the phosphorylation/inhibition events are marked by biotinylated anti-phosphoserinene/anti-phosphotyrosine antibodies. silver deposited gold nanoparticles are employed as active sers substrates by attaching biotin-conjugated gold nanoparticles to the antibodies through avidin bridges. the avidin conjugated fluorescein is used as sers probe and corresponding sers spectra have been collected from different spots on the microarray for addressing the activity of kinase. among the 80 compounds, we have identified 2 inhibitors for pka and 9 inhibitors for lck. in addition, the half maximal inhibitory concentration (i.e., ic(50) value) and the inhibiting type (type i) of specific inhibitor have also been determined which agree with literature reported results.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000292164200032
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44742]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li T,Liu DJ,Wang ZX. Screening kinase inhibitors with microarray-based Raman spectroscopic assay[J]. analytical methods,2011,3(4):1003-1007.
APA	Li T,Liu DJ,&Wang ZX.(2011).Screening kinase inhibitors with microarray-based Raman spectroscopic assay.analytical methods,3(4),1003-1007.
MLA	Li T,et al."Screening kinase inhibitors with microarray-based Raman spectroscopic assay".analytical methods 3.4(2011):1003-1007.