Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS

	Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS
	Yang HM ; Yu YN ; Song FR ; Liu SY
刊名	journal of the american society for mass spectrometry
	2011
卷号	22 期号:5 页码:845-855
关键词	DESORPTION/IONIZATION MASS-SPECTROMETRY COLLISION-INDUCED DISSOCIATION HUMAN-MILK OLIGOSACCHARIDES NEGATIVE-ION MODE UNDERIVATIZED OLIGOSACCHARIDES DESORPTION IONIZATION LINKED GLYCANS FRAGMENTATION GLYCOPROTEIN
ISSN号	1044-0305
通讯作者	liu sy
中文摘要	maldi in-source decay (isd) technique described to date has proven to be a convenient and rapid method for sequencing purified peptides and proteins. however, the general isd still can not produce adequate fragments for the detailed structural elucidation of oligosaccharides. in this study, an efficient and practical method termed the laser-enhanced isd (leisd) technique of maldi-fticr ms allows highly reliable and abundant fragmentation of the neutral oligosaccharides, which was attributed to the ultrahigh irradiation laser of mj level. the yield of isd fragmentation was evaluated under different laser powers for 7 neutral oligosaccharides using dhb as matrix. better quality isd spectra including fragment ions in low-mass region were obtained at higher laser power. results from the leisd of oligosaccharides demonstrated that a significantly better signal-to-noise ratio (s/n) and more structural information could be obtained in comparison to the conventional cid. it was also suggested that the valuable a ions derived from cross-ring cleavage of the linear oligosaccharides allowed the distinction among alpha(1 -> 4)-, alpha(1 -> 6)-, beta(1 -> 4)-, and beta(1 -> 3)-linked isobaric structures according to fragment types and intensities. in addition, ideal fragmentation ions observed by leisd method facilitated the determination of the sequences and branched points of complex oligosaccharides from human milk.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000290454300006
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44676]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Yang HM,Yu YN,Song FR,et al. Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS[J]. journal of the american society for mass spectrometry,2011,22(5):845-855.
APA	Yang HM,Yu YN,Song FR,&Liu SY.(2011).Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS.journal of the american society for mass spectrometry,22(5),845-855.
MLA	Yang HM,et al."Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS".journal of the american society for mass spectrometry 22.5(2011):845-855.