Cell-based biosensor for measurement of phenol and nitrophenols toxicity

	Cell-based biosensor for measurement of phenol and nitrophenols toxicity
	Liu C ; Yong DM ; Yu DB ; Dong SJ
刊名	talanta
	2011
卷号	84 期号:3 页码:766-770
关键词	BIOCHEMICAL OXYGEN-DEMAND ELECTROCHEMICAL MEASUREMENT RESPIRATION INHIBITION ACTIVATED-SLUDGE MICROTOX MICREDOX ASSAY
ISSN号	0039-9140
通讯作者	dong sj
中文摘要	a cost-effective whole cell biosensor based on electrochemical technique to detect toxicities of phenol and nitrophenols has been developed. this method relied on the inhibition effect for respiratory chain activity of microorganism by toxicant, which was measured by chronoamperometry using mediator (ferricyanide). the current signals produced by suspended microorganisms and reoxidation of ferrocyanide were transformed to inhibiting efficiency directly, and 50% inhibiting concentration (ic(50)) was chosen as the quantitative standard of toxicity. the test microorganisms used here consist of three bacilli (escherichia coli, enterobacter cloacae and alcaligenes faecalis), two pseudomonas (pseudomonas fluorescens and pseucomonas putida) and one fungus (trichosporon cutaneum). 3,5-dichlorophenol (dcp) was taken as the reference toxicant. the results showed that the microorganisms which belong to the same bacterial family had similar trends of inhibitions on respiratory activity and similar ic50 values. by comparing the ic(50) values, p. fluorescens was the most sensitive one to dcp toxicity, its ic(50) was estimated to be 4.2 mg/l. ph 7.0 and together with the standard glucose-glutamic acid (gga) as an exogenous material were taken for optimum conditions in this study. here. p. fluorescens as model test microorganism was employed to assess toxicities of phenol and nitrophenols under the optimum conditions. ic(50) values of 291.4 mg/l for phenol, 64.1 mg/l for 2-np, 71.4 mg/l for 3-np and 14.0 mg/l for 4-np were determined at 60 min, respectively. comparison with the results of published data has confirmed that this cell biosensor is a sensitive and rapid alternative to toxicity screening of chemicals. crown copyright (c) 2011 published by elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000290187200025
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44631]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liu C,Yong DM,Yu DB,et al. Cell-based biosensor for measurement of phenol and nitrophenols toxicity[J]. talanta,2011,84(3):766-770.
APA	Liu C,Yong DM,Yu DB,&Dong SJ.(2011).Cell-based biosensor for measurement of phenol and nitrophenols toxicity.talanta,84(3),766-770.
MLA	Liu C,et al."Cell-based biosensor for measurement of phenol and nitrophenols toxicity".talanta 84.3(2011):766-770.