Label-free colorimetric immunoassay for the simple and sensitive detection of neurogenin3 using gold nanoparticles

	Label-free colorimetric immunoassay for the simple and sensitive detection of neurogenin3 using gold nanoparticles
	Yuan Y ; Zhang J ; Zhang HC ; Yang XR
刊名	biosensors & bioelectronics
	2011
卷号	26 期号:10 页码:4245-4248
关键词	OPTICAL-PROPERTIES PANCREAS REVEALS ASSAY
ISSN号	0956-5663
通讯作者	zhang hc
中文摘要	neurogenin3 (ngn3), as a marker for pancreatic endocrine precursor cells and an essential ingredient in the development of islet cells, was quantitatively detected for the first time. based on a non-cross-linking specific interaction mechanism, a label-free colorimetric immunoassay for the synthetic peptide fragment of ngn3 (skqrrsrrkkand) using glutathione (-glu-cys-gly, gsh) functionalized gold nanoparticles (gnps) is reported. the anti-ngn3 antibody conjugated gnps (gnp-ab) was formed through electrostatic interaction upon the addition of anti-ngn3 antibody to the gsh-modified gnps solution. monobinding of the positively charged ngn3 to the negatively charged gnp-ab will minimize the electrostatic repulsion between nanoparticles by neutralizing the surface charge, and then agglomeration is induced by an increasing salt concentration. under the optimal conditions, the assay showed a linear response range of 50-300 ng/ml for the peptide with a detection limit being 20 ng/ml. the preliminary study on ngn3 opens up an innovative insight to detect short synthetic peptide fragment of antigen, and may own an opportunity for practical applications in clinical diagnosis and therapeutics. (c) 2011 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000291904000044
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44569]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Yuan Y,Zhang J,Zhang HC,et al. Label-free colorimetric immunoassay for the simple and sensitive detection of neurogenin3 using gold nanoparticles[J]. biosensors & bioelectronics,2011,26(10):4245-4248.
APA	Yuan Y,Zhang J,Zhang HC,&Yang XR.(2011).Label-free colorimetric immunoassay for the simple and sensitive detection of neurogenin3 using gold nanoparticles.biosensors & bioelectronics,26(10),4245-4248.
MLA	Yuan Y,et al."Label-free colorimetric immunoassay for the simple and sensitive detection of neurogenin3 using gold nanoparticles".biosensors & bioelectronics 26.10(2011):4245-4248.