EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging

	EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging
	Liu TJ ; Liu S ; Hu XL ; Sheng SH ; Huang YB ; Jing XB
刊名	chemical research in chinese universities
	2011
卷号	27 期号:4 页码:628-634
关键词	DRUG-DELIVERY SYSTEMS CANCER CONJUGATE NANOCAPSULES BIODISTRIBUTION NANOPARTICLES THERAPEUTICS
ISSN号	1005-9040
通讯作者	liu tj
中文摘要	enhanced permeation and retention(epr) targeting effect of rhodamine b labeled peg-b-p(la-co-dhp) [peg: poly(ethylene glycol); la: l-lactide; dhp: 2,2-dihydroxylmethyl-propylene carbonate] micelles(rhb-micelles) was observed in h22 liver cancer bearing mice. the rhb-micelles were prepared by conjugating rhodamine b with the dhp units of amphiphilic block copolymer peg-b-p(la-co-dhp) followed by subsequent self-assembling of the conjugate. the parent copolymer peg-b-p(la-co-dhp) was synthesized by ring-opening copolymerization of la and dhp with peg as macroinitiator and diethyl zinc(znet(2)) as catalyst. the micelles have a spherical shape and the average diameter is ca. 50 nm by tem(transmission electron microscope) or 80 nm by dls(dynamic light scattering). their in vitro cell uptake experiment by clsm(confocal laser scanning microscopy) and flow cytometry showed preferential internalization of micelles by mcf-7 human breast cancer cells to free rhb. the in vivo tests by live animal imaging and ex vivo excised organ imaging showed that after vena tail injection, free rhb molecules were distributed in the whole body through the circulation system and then gradually metabolized and excreted and there was no preferential partition in tumor bed from the beginning to the end. but the rhb-micelles were preferentially distributed to the tumor bed so that their concentration(fluorescent intensity) in tumor bed got the level of the liver at a certain time point between 1 and 6 h and reached a maximum relative intensity at around 12 h, indicating an obvious epr effect of rhb-micelles in h22 liver cancer.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000294379100023
公开日期	2012-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44496]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Liu TJ,Liu S,Hu XL,et al. EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging[J]. chemical research in chinese universities,2011,27(4):628-634.
APA	Liu TJ,Liu S,Hu XL,Sheng SH,Huang YB,&Jing XB.(2011).EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging.chemical research in chinese universities,27(4),628-634.
MLA	Liu TJ,et al."EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging".chemical research in chinese universities 27.4(2011):628-634.