A simple and sensitive fluorescent sensing platform for Hg(2+) ions assay based on G-quenching

	A simple and sensitive fluorescent sensing platform for Hg(2+) ions assay based on G-quenching
	Hu P ; Jin LH ; Zhu CZ ; Dong SJ
刊名	talanta
	2011
卷号	85 期号:1 页码:713-717
关键词	COLORIMETRIC DETECTION GOLD NANOPARTICLES MERCURY(II) IONS DNA-HAIRPINS DYES OLIGONUCLEOTIDES MOLECULES MECHANISM TRANSPORT PROBES
ISSN号	0039-9140
通讯作者	dong sj
中文摘要	in this work, a novel fluorescence biosensor was demonstrated for detection of hg(2+) ions with relatively high selectivity and sensitivity. the sensing scheme was based on g-quenching induced by hg(2+) ions. in the presence of hg(2+) ions, the single-stranded signal probe which has carboxylfluorescein (fam) and guanine segment at its 5' and 3' ends, respectively, folded into duplex-like structure via the hg(2+)-mediated coordination of t-hg(2+)-t base pairs. it brought guannine segment close to the dye and caused a remarkable decrease of fluorescence signal. the sensor showed a sensitive response to hg(2+) ions in a concentration range from 0.5 to 10 mu m. and a detection limit of 0.5 nm was given. this homogeneous system required only a single-labeled oligonucleotide, operated by concise procedures, and possessed comparable sensitivity as previous approaches. furthermore, the sensor exhibits a great perspective for future practical applications. (c) 2011 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
公开日期	2012-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44466]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Hu P,Jin LH,Zhu CZ,et al. A simple and sensitive fluorescent sensing platform for Hg(2+) ions assay based on G-quenching[J]. talanta,2011,85(1):713-717.
APA	Hu P,Jin LH,Zhu CZ,&Dong SJ.(2011).A simple and sensitive fluorescent sensing platform for Hg(2+) ions assay based on G-quenching.talanta,85(1),713-717.
MLA	Hu P,et al."A simple and sensitive fluorescent sensing platform for Hg(2+) ions assay based on G-quenching".talanta 85.1(2011):713-717.