Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing

	Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing
	Hu YW ; Hua SC ; Li FH ; Jiang YY ; Bai XX ; Li D ; Niu L
刊名	biosensors & bioelectronics
	2011
卷号	26 期号:11 页码:4355-4361
关键词	IONIC LIQUID PAT GENE ELECTRODES SENSOR COMPOSITE PROBE OXIDE ACID FILM SENSITIVITY
ISSN号	0956-5663
通讯作者	hua sc
中文摘要	sensitive electrochemical impedance assay of dna hybridization by using a novel graphene sheets platform was achieved. the graphene sheets were firstly functionalized with 3,4,9,10-perylene tetracarboxylic acid (pica). pica molecules separated graphene sheets efficiently and introduced more negatively-charged -cooh sites, both of which were beneficial to the decoration of graphene with gold nanoparticles. then amine-terminated ionic liquid (nh(2)-il) was applied to the reduction of haucl(4) to gold nanoparticles. the green-synthesized gold nanoparticles, with the mean diameter of 3 nm, dispersed uniformly on graphene sheets and its outer layer was positively charged imidazole termini. due to the presence of large graphene sheets and nh(2)-il protected gold nanoparticles, dna probes could be immobilized via electrostatic interaction and adsorption effect. electrochemical impedance value increased after dna probes immobilization and hybridization, which was adopted as the signal for label-free dna hybridization detection. unlike previously anchoring dna to gold nanoparticles, this label-free method was simple and noninvasive. the conserved sequence of the pot gene of human immunodeficiency virus 1 was satisfactorily detected via this strategy. (c) 2011 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000293104100016
公开日期	2012-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44465]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Hu YW,Hua SC,Li FH,et al. Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing[J]. biosensors & bioelectronics,2011,26(11):4355-4361.
APA	Hu YW.,Hua SC.,Li FH.,Jiang YY.,Bai XX.,...&Niu L.(2011).Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing.biosensors & bioelectronics,26(11),4355-4361.
MLA	Hu YW,et al."Green-synthesized gold nanoparticles decorated graphene sheets for label-free electrochemical impedance DNA hybridization biosensing".biosensors & bioelectronics 26.11(2011):4355-4361.