Differential Expression of Total Proteins of Human Ovarian Cancer COC1 Cell Line Treated by cis-Diamminedichloroplatinum (DDP)

	Differential Expression of Total Proteins of Human Ovarian Cancer COC1 Cell Line Treated by cis-Diamminedichloroplatinum (DDP)
	Guan SL ; Lu L ; Hu XL ; Liu ZY ; Song FR ; Liu ZQ
刊名	acta chimica sinica
	2011
卷号	69 期号:14 页码:1721-1724
关键词	TUMORIGENESIS PROHIBITIN
ISSN号	0567-7351
通讯作者	guan sl
中文摘要	researcher cultured cells in vitro, when the concentration of cells is 10(9)/ml, gained the total proteins within cells, separated the total-proteins by using two-dimensions electrophorese sds-page (sds polyacrylamide gel electrophoresis), and analyzed the electrophoretogram with pdquest 7.1.1 software, then detcted the mass by matrix-assisted laser desorption/ionization mass spectrometry (maldi-tof-ms) after in-gel digestion protocol, finally, identified the expressing proteins betweem ovarian cancer parent cell coc1 and subline treated by cisplatin, to search for the molecular markers of ovarian cancer cell in the phases of cytogenesis, growth and apoptosis. resultly, 11 proteins had matches returned from the database search leading to the identification of these proteins, only about 7 proteins [all for the ovarian cancer cell coc1 treated by ddp (cis-diamminedichloroplatinum)] have a significant difference among these proteins. meanwhile, 4 proteins are different expression on level, 5 proteins are molecular chaperones, 4 proteins are relative to energy metabolism. function analysis demonstrate the expression diffence, these different proteins profit to study the bio-marker of ovairian cancer.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000294375100018
公开日期	2012-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44451]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Guan SL,Lu L,Hu XL,et al. Differential Expression of Total Proteins of Human Ovarian Cancer COC1 Cell Line Treated by cis-Diamminedichloroplatinum (DDP)[J]. acta chimica sinica,2011,69(14):1721-1724.
APA	Guan SL,Lu L,Hu XL,Liu ZY,Song FR,&Liu ZQ.(2011).Differential Expression of Total Proteins of Human Ovarian Cancer COC1 Cell Line Treated by cis-Diamminedichloroplatinum (DDP).acta chimica sinica,69(14),1721-1724.
MLA	Guan SL,et al."Differential Expression of Total Proteins of Human Ovarian Cancer COC1 Cell Line Treated by cis-Diamminedichloroplatinum (DDP)".acta chimica sinica 69.14(2011):1721-1724.