Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe

	Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe
	Wang XH ; Wu L ; Ren JS ; Miyoshi D ; Sugimoto N ; Qu XG
刊名	biosensors & bioelectronics
	2011
卷号	26 期号:12 页码:4804-4809
关键词	CYCLIN-A EXPRESSION UNMODIFIED GOLD NANOPARTICLES SILVER NANOPARTICLES CELL-PROLIFERATION MOLECULAR BEACONS CARBON NANOTUBES DNA-REPLICATION K562 CELLS ASSAY A(2)
ISSN号	0956-5663
通讯作者	qu xg
中文摘要	assays for non-enzyme protein based on peptide-protein interaction are few due to the fact that most of peptide-protein bindings do not produce easily measurable output signals. here we report a homogenous assay for colorimetric and quantitative detection of a cancer marker and promising antitumor target, cyclin a(2), using noncrosslinking aggregation of unmodified aunps/agnps by utilizing the difference of coagulating ability of a cationic peptide probe (p1) and its binding form toward naked aunps/agnps. in the absence of cyclin a(2), p1 coagulates particles immediately, whereas cyclin a(2) binding prevents the interaction of p1 with metal particles surface, significantly reducing the magnitude of aggregation. the extent of aggregation is dependent on the concentration of the target protein cyclin a(2) and the difference in color can readily be distinguished by spectrometer and naked eyes. the assay is sensitive and selective. cyclin a(2) assay using aunps as colorimetric indicator is more easily monitored by naked eyes owing to the distinct color change, and 40 nm cyclin a(2) can be detected without the aid of any instruments. using inexpensive desktop spectrometer, cyclin a(2) assay using agnps as colorimetric indicator can detect as low as 30 nm cyclin a(2), which is 20 fold lower than that of cyclin a(2) assay using terbium-chelating peptide as the probe reported recently (pazos et al., 2008, 130, 9652-9653). this strategy will shed light on developing of unlabeled peptide-based protein biosensors. (c) 2011 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000293932300026
公开日期	2012-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44403]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang XH,Wu L,Ren JS,et al. Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe[J]. biosensors & bioelectronics,2011,26(12):4804-4809.
APA	Wang XH,Wu L,Ren JS,Miyoshi D,Sugimoto N,&Qu XG.(2011).Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe.biosensors & bioelectronics,26(12),4804-4809.
MLA	Wang XH,et al."Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe".biosensors & bioelectronics 26.12(2011):4804-4809.