Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery

	Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery
	Lee Y ; Lee SH ; Kim JS ; Maruyama A ; Chen XS ; Park TG
刊名	journal of controlled release
	2011
卷号	155 期号:1 页码:3-10
关键词	QUANTUM DOTS GENE DELIVERY DNA COMPLEXES POLYMERS DESIGN CELLS NANOCRYSTALS THERAPEUTICS CYTOTOXICITY POLYPLEXES
ISSN号	0168-3659
通讯作者	park tg
中文摘要	development of nano-sized gene delivery vehicles for small interfering rna (sirna) delivery is of great importance for their clinical applications such as cancer therapy. herein, we demonstrate the controlled synthesis of polyethyleneimine (pei)-coated gold nanoparticles (aunps) using catechol-conjugated pei (pei-c) for sirna delivery. since the conjugated catechol groups are reductive and moderately hydrophobic. pei-c formed spherical multi-cored micelles in aqueous solution and served as reductive templates for the growth and synthesis of spherical aunps with tunable sizes and surface charges. pei-c was stably anchored on the surface of growing crystal gold seeds with crosslinking, resulting in robust cationic aunps. the fabricated pei-coated aunps formed stable complexes with sirna, and the complexes showed an excellent gene silencing effect in cancer cells. size and surface charge values of the synthesized aunps had a great influence on intracellular uptake and unpacking of sirna, and the resultant gene silencing efficiency. the pei-coated aunps exhibited an extremely low cytotoxicity due to the reduced density of primary amine groups and the absence of uncomplexed pei fraction in aqueous solution. (c) 2010 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000295998200003
公开日期	2012-06-08
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44295]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Lee Y,Lee SH,Kim JS,et al. Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery[J]. journal of controlled release,2011,155(1):3-10.
APA	Lee Y,Lee SH,Kim JS,Maruyama A,Chen XS,&Park TG.(2011).Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery.journal of controlled release,155(1),3-10.
MLA	Lee Y,et al."Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery".journal of controlled release 155.1(2011):3-10.