Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection | |
Yang X ; Li T ; Li BL ; Wang EK | |
刊名 | analyst |
2010 | |
卷号 | 135期号:1页码:71-75 |
关键词 | COLORIMETRIC DETECTION PEROXIDASE-ACTIVITY AMPLIFIED ANALYSIS DNAZYME ION THROMBIN APTAMERS SENSOR OLIGONUCLEOTIDE NANOPARTICLES |
ISSN号 | 0003-2654 |
通讯作者 | wang ek |
中文摘要 | a novel visual method for k+ detection is developed utilizing a k+-sensitive g-quadruplex dna named ps5.m as the sensing element. in the absence of k+, ps5.m is in a random coil state and does not bind hemin. upon addition of k+, ps5.m folds into the quadruplex structure stabilized by k+. such quadruplex structure is able to bind hemin to form the hemin-g-quadruplex dnazyme that catalyzes the h2o2-mediated oxidation of colorless 3,3',5,5'-tetramethylbenzidine to the blue product. under the optimal conditions, the color change can be clearly observed with the naked eye in the concentration range from 2 to 1000 mm. through this facile approach, k+ can be detected down to 2 mu m, with high selectivity against other cations. in view of its simplicity, sensitivity and specificity, our developed visual method for k+ detection would potentially be utilized for bioassays and k+-sensitive test paper. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000272711400008 |
公开日期 | 2012-06-07 |
内容类型 | 期刊论文 |
源URL | [http://ir.ciac.jl.cn/handle/322003/43995] |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Yang X,Li T,Li BL,et al. Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection[J]. analyst,2010,135(1):71-75. |
APA | Yang X,Li T,Li BL,&Wang EK.(2010).Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection.analyst,135(1),71-75. |
MLA | Yang X,et al."Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection".analyst 135.1(2010):71-75. |
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