Lead(II)-Induced Allosteric G-Quadruplex DNAzyme as a Colorimetric and Chemiluminescence Sensor for Highly Sensitive and Selective Pb2+ Detection

	Lead(II)-Induced Allosteric G-Quadruplex DNAzyme as a Colorimetric and Chemiluminescence Sensor for Highly Sensitive and Selective Pb2+ Detection
	Li T ; Wang E ; Dong S
刊名	analytical chemistry
	2010
卷号	82 期号:4 页码:1515-1520
关键词	PEROXIDASE-ACTIVITY GOLD NANOPARTICLES AMPLIFIED DETECTION TELOMERASE ACTIVITY CATALYTIC DNA LEAD IONS LABEL CHEMOSENSORS BIOSENSOR APTAMER
ISSN号	0003-2700
通讯作者	wang e
中文摘要	the lead ion (pb2+) has been proven to induce a conformational change of k-stabilized g-quadruplex dnazyme and inhibit the peroxidase-like activity [li, t.; wang, e.; dong, s. j. am. chem. soc. 2009, 131, 15082-15083]. ibis provides a rationale for utilizing pb2+-induced allosteric g-quadruplex dnazyme to probe aqueous pb2+. here, we choose a common g-quadruplex dnazyme named ps2.m to develop a novel pb2+ sensor with two detection means: colorimetry and chemiluminescence (cl). in the presence of k+, ps2.m (with hemin as a cofactor) exhibits a superior dnazyme activity and effectively catalyzes the h2o2-mediated oxidation of 2,2'-azino-bis(3-ethylben-zothiazoline-6-sulfonic acid) diammonium salt (abts) or luminol, which results in a color change or generates cl emission. upon the addition of pbl(2+), k-stabilized ps2.m is induced to convert to the pb2+-stabilized structure with higher stability but lower dnazyme activity, which is reflected by an obvious increase in dna melting temperature but a sharp decrease in readout signal. this allows us to utilize ps2.m for quantitative analysis of aqueous pb2+ using the abts-h2o2 colorimetric system and luminol-h2o2 cl system. in each case, the readout signal is linearly dependent on the logarithm of pb2+ concentration within a certain range. nevertheless, two sensing systems provide different sensitivity for ph2+ analysis. with colorimetry, pb2+ can be detected at a level of 32 nm (similar to 7 ppb), whereas the detection limit of pb2+ is 1 nm (0.2 ppb) when utilizing the cl method. in addition to high sensitivity, the above sensing systems exhibit good selectivity for pb2+ over other metal ions. these results demonstrate the facility and effectivity of our introduced dnazyme-based sensor for quantitative pb2+ analysis.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000274466100048
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43925]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li T,Wang E,Dong S. Lead(II)-Induced Allosteric G-Quadruplex DNAzyme as a Colorimetric and Chemiluminescence Sensor for Highly Sensitive and Selective Pb2+ Detection[J]. analytical chemistry,2010,82(4):1515-1520.
APA	Li T,Wang E,&Dong S.(2010).Lead(II)-Induced Allosteric G-Quadruplex DNAzyme as a Colorimetric and Chemiluminescence Sensor for Highly Sensitive and Selective Pb2+ Detection.analytical chemistry,82(4),1515-1520.
MLA	Li T,et al."Lead(II)-Induced Allosteric G-Quadruplex DNAzyme as a Colorimetric and Chemiluminescence Sensor for Highly Sensitive and Selective Pb2+ Detection".analytical chemistry 82.4(2010):1515-1520.