Screening Kinase Inhibitors with a Microarray-Based Fluorescent and Resonance Light Scattering Assay

	Screening Kinase Inhibitors with a Microarray-Based Fluorescent and Resonance Light Scattering Assay
	Li T ; Liu DJ ; Wang ZX
刊名	analytical chemistry
	2010
卷号	82 期号:7 页码:3067-3072
关键词	GOLD NANOPARTICLE PROBES PEPTIDE ARRAYS PROTEIN SPECIFICITY DESIGN PHOSPHORYLATION PROTEOMICS POTENT TOOLS
ISSN号	0003-2700
通讯作者	wang zx
中文摘要	in this technical note, a microarray-based spectroscopic assay with two readout principles, fluorescence and resonance light scattering (rls), for screening kinase inhibitors has been reported. in this assay, the phosphorylation and inhibition events are marked by biotinylated antiphosphoserinen/antiphosphotyrosine antibodies, and gold nanoparticles are attached to the antibodies by standard avidin-biotin chemistry followed by silver deposition for his signal enhancement. the avidin conjugated fluorescein is used as a fluorescent probe. assays for both serine kinase, the alpha-catalytic subunit of cyclic adenosine 5'-monophosphate (camp) dependent protein kinase (pka), and tyrosine kinase, leukocyte-specific protein tyrosine kinase (lck), have been developed. the utility of this assay to high-throughput screening was demonstrated with a commercial inhibitor library, a collection of 80 kinase inhibitors, and satisfactory results were obtained. in addition, quantitative determination of binding strength and the inhibiting type (type i) of these inhibitors are also demonstrated by the adenosine 5'-triphosphate (atp) competing assays.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000276004000064
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43835]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li T,Liu DJ,Wang ZX. Screening Kinase Inhibitors with a Microarray-Based Fluorescent and Resonance Light Scattering Assay[J]. analytical chemistry,2010,82(7):3067-3072.
APA	Li T,Liu DJ,&Wang ZX.(2010).Screening Kinase Inhibitors with a Microarray-Based Fluorescent and Resonance Light Scattering Assay.analytical chemistry,82(7),3067-3072.
MLA	Li T,et al."Screening Kinase Inhibitors with a Microarray-Based Fluorescent and Resonance Light Scattering Assay".analytical chemistry 82.7(2010):3067-3072.