Preparation of cell membranes for high resolution imaging by AFM

	Preparation of cell membranes for high resolution imaging by AFM
	Wang HD ; Hao X ; Shan YP ; Jiang JG ; Cai MJ ; Shang X
刊名	ultramicroscopy
	2010
卷号	110 期号:4 页码:305-312
关键词	ATOMIC-FORCE MICROSCOPY SINGLE-MOLECULE MICROSCOPY SOLID-STATE NMR PLASMA-MEMBRANE LIPID-BILAYERS LEWIS-X PEPTIDE DNA TRISACCHARIDE CONFORMATION
ISSN号	0304-3991
通讯作者	wang hd
中文摘要	studies of cell membrane structure by atomic force microscopy (afm) have been limited because of the softness of cell membranes. here, we utilize a new technique of sample preparation to lay red blood cell membranes on the top of a mica surface to obtain high resolution images by in-situ afm on both sides of cell membranes. our results indicate that the location of oligosaccharides and proteins in red blood cell membranes might be different from the current membrane model. the inner membrane leaflet is covered by dense proteins with fewer free lipids than expected. in contrast, the outer membrane leaflet is quite smooth; oligosaccharides and peptides supposed to protrude out of the outer membrane leaflet surface might be actually hidden in the middle of hydrophilic lipid heads; transmembrane proteins might form domains in the membranes revealed by pngase f and trypsin digestion. our result could be significant to interpret some functions about red blood cell membranes and guide to heal the blood diseases related to cell membranes. (c) 2009 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000276290200005
公开日期	2012-06-07
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43812]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang HD,Hao X,Shan YP,et al. Preparation of cell membranes for high resolution imaging by AFM[J]. ultramicroscopy,2010,110(4):305-312.
APA	Wang HD,Hao X,Shan YP,Jiang JG,Cai MJ,&Shang X.(2010).Preparation of cell membranes for high resolution imaging by AFM.ultramicroscopy,110(4),305-312.
MLA	Wang HD,et al."Preparation of cell membranes for high resolution imaging by AFM".ultramicroscopy 110.4(2010):305-312.