A carbon nanotubes based ATP apta-sensing platform and its application in cellular assay

	A carbon nanotubes based ATP apta-sensing platform and its application in cellular assay
	Zhang LB ; Wei H ; Li J ; Li T ; Li D ; Li YH ; Wang EK
刊名	biosensors & bioelectronics
	2010
卷号	25 期号:8 页码:1897-1901
关键词	MOLECULAR RECOGNITION DNA HYBRIDIZATION FLUORESCENCE ADENOSINE SENSOR NANOPARTICLES HELIX
ISSN号	0956-5663
通讯作者	wang ek
中文摘要	in this paper, a sensitive and selective fluorescent aptasensor for adenosine triphosphate (atp) detection is constructed, based on the noncovalent assembly of dye-labeled atp aptamer and single-walled carbon nanotubes (swnts). in the absence of atp, the dye tethered to the atp aptamer is close to swnts, which can effectively quench fluorescence of the dye. upon adding atp, the fluorophore keeps away from the quencher, since atp specifically binds to the aptamer and competes with carbon nanotubes, resulting in an increase in the fluorescence intensity. this enables atp to be detected down to 4.5 nm. to the best of our knowledge, this is the most sensitive fluorescent atp aptasensor. in addition, prominent fluorescence signals were obtained in cellular atp assays, thus the aptasensor could be used to detect atp in real samples. (c) 2010 elsevier b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000276424200006
公开日期	2012-06-06
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43806]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhang LB,Wei H,Li J,et al. A carbon nanotubes based ATP apta-sensing platform and its application in cellular assay[J]. biosensors & bioelectronics,2010,25(8):1897-1901.
APA	Zhang LB.,Wei H.,Li J.,Li T.,Li D.,...&Wang EK.(2010).A carbon nanotubes based ATP apta-sensing platform and its application in cellular assay.biosensors & bioelectronics,25(8),1897-1901.
MLA	Zhang LB,et al."A carbon nanotubes based ATP apta-sensing platform and its application in cellular assay".biosensors & bioelectronics 25.8(2010):1897-1901.