Exploring the bZIP transcription factor regulatory network in Neurospora crassa

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	Exploring the bZIP transcription factor regulatory network in Neurospora crassa
	Tian, Chaoguang 1,2; Li, Jingyi 1; Glass, N. Louise 1
刊名	MICROBIOLOGY-SGM
	2011-03-01
卷号	157 期号:3 页码:747-759
英文摘要	Transcription factors (TFs) are key nodes of regulatory networks in eukaryotic organisms, including filamentous fungi such as Neurospora crassa. The 178 predicted DNA-binding TFs in N. crassa are distributed primarily among six gene families, which represent an ancient expansion in filamentous ascomycete genomes; 98 TF genes show detectable expression levels during vegetative growth of N. crassa, including 35 that show a significant difference in expression level between hyphae at the periphery versus hyphae in the interior of a colony. Regulatory networks within a species genome include paralogous TFs and their respective target genes (TF regulon). To investigate TF network evolution in N. crassa, we focused on the basic leucine zipper (bZIP) IF family, which contains nine members. We performed baseline transcriptional profiling during vegetative growth of the wild-type and seven isogenic, viable bZIP deletion mutants. We further characterized the regulatory network of one member of the bZIP family, NCU03905. NCU03905 encodes an Ap1-like protein (NcAp-1), which is involved in resistance to multiple stress responses, including oxidative and heavy metal stress. Relocalization of NcAp-1 from the cytoplasm to the nucleus was associated with exposure to stress. A comparison of the NcAp-1 regulon with Ap1-like regulons in Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Aspergillus fumigatus showed both conservation and divergence. These data indicate how N. crassa responds to stress and provide information on pathway evolution.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	DNA-DAMAGE RESPONSE ; AMINO-ACID CONTROL ; OXIDATIVE STRESS ; CANDIDA-ALBICANS ; SACCHAROMYCES-CEREVISIAE ; MULTIPLE PATHWAYS ; SYSTEMS-APPROACH ; GENOME SEQUENCE ; BINDING PROTEIN ; GENE-EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000288833000014
公开日期	2011-08-16
内容类型	期刊论文
源URL	[http://localhost/handle/0/77]
专题	天津工业生物技术研究所_微生物功能基因组学与生物技术实验室田朝光_期刊论文
作者单位	1.Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Tian, Chaoguang,Li, Jingyi,Glass, N. Louise. Exploring the bZIP transcription factor regulatory network in Neurospora crassa[J]. MICROBIOLOGY-SGM,2011,157(3):747-759.
APA	Tian, Chaoguang,Li, Jingyi,&Glass, N. Louise.(2011).Exploring the bZIP transcription factor regulatory network in Neurospora crassa.MICROBIOLOGY-SGM,157(3),747-759.
MLA	Tian, Chaoguang,et al."Exploring the bZIP transcription factor regulatory network in Neurospora crassa".MICROBIOLOGY-SGM 157.3(2011):747-759.