Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam

CORC > 天津工业生物技术研究所 > 功能糖与天然活性物质研究组孙媛霞 > 期刊论文

	Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam
	Sun, Yuanxia 1; Hayakawa, Shigeru 2; Ogawa, Masahiro 2; Naknukool, Supaporn 2; Guan, Yupin 1; Matsumoto, Yoshiyuki 2
刊名	FOOD SCIENCE AND BIOTECHNOLOGY
	2011-04-01
卷号	20 期号:2 页码:303-310
关键词	angiotensin I-converting enzyme (ACE) inhibitor bioactive peptide hydrolysis dried mantle freshwater clam
英文摘要	Solid wastes of freshwater clam in food processing, including mainly mantle, were used as a raw material for the recovery of bioactive peptides related to angiotensin I-converting enzyme (ACE) inhibitor. Among the primary hydrolysates of dried mantle (DM), the peptides corresponding to hydrolysates using 2 crude peptidases exhibited a strong ACE inhibitory activity (IC(50), 0.23 mg/mL), and recovery efficiency of soluble materials and their protein content were considerably large with 42.65% and 468.6 mg/g, respectively. The ACE inhibitory activity of all secondary hydrolysates digested by pepsin and trypsin was significantly increased as compared to primary hydrolysates. Furthermore, the peptic secondary hydrolysates were fractionated by gel filtration and reverse phase-HPLC (RP-HPLC) and characterized by matrix-assisted laser desorption-ionization time of flight/mass spectrometry (MALDI-TOF/MS). These peptides with molecular weight of less than 1 kDa possessed the stronger ACE inhibitory effect, and their inhibitory pattern was found to be competitive. The results showed that the DM hydoplysates might be utilized as a rich source of bioactive peptide.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Food Science & Technology
研究领域[WOS]	Food Science & Technology
关键词[WOS]	SPECTROPHOTOMETRIC ASSAY ; FRAME PROTEIN ; PEPTIDES ; MUSCLE ; COLLAGEN ; SCALES ; MILK ; SKIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000290039500003
公开日期	2011-08-01
内容类型	期刊论文
源URL	[http://localhost/handle/0/57]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Seoul, Peoples R China 2.Kagawa Univ, Dept Biochem & Food Sci, Kagawa 7610795, Japan
推荐引用方式 GB/T 7714	Sun, Yuanxia,Hayakawa, Shigeru,Ogawa, Masahiro,et al. Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2011,20(2):303-310.
APA	Sun, Yuanxia,Hayakawa, Shigeru,Ogawa, Masahiro,Naknukool, Supaporn,Guan, Yupin,&Matsumoto, Yoshiyuki.(2011).Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam.FOOD SCIENCE AND BIOTECHNOLOGY,20(2),303-310.
MLA	Sun, Yuanxia,et al."Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam".FOOD SCIENCE AND BIOTECHNOLOGY 20.2(2011):303-310.