Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs

CORC > 金属研究所 > 中国科学院金属研究所

	Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs
	Y. Q. Guan ; Z. B. Li ; J. M. Chen ; H. M. Tao ; W. W. Wang ; Z. Zheng ; L. Li ; J. M. Liu
刊名	Biomaterials
	2011
卷号	32 期号:14 页码:3637-3646
关键词	Co-immobilized IFN-gamma plus TNF-alpha Polystyrene material HeLa EndoG tumor-necrosis-factor nf-kappa-b ifn-gamma interferon-gamma endonuclease-g factor-alpha apoptosis activation micelles p53
ISSN号	0142-9612
中文摘要	Synthesis of anticancer polymeric materials plus their biological applications is one of the most charming and active research areas in biological functional materials. However, the predominant mechanisms for controlling cancer cell viability are not yet clear. In this work, cell culture polymeric materials co-immobilized with death signal proteins interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) on the surface were prepared by photochemical method to develop an anticancer polymeric drug model. Various characterizations on the microstructures and compositions, including the Fourier transform infrared spectroscopy, UV absorption spectroscopy, fluorescence measurement, atomic force microscopy, and electron spectroscopy for chemical analysis, were performed. For addressing the biological applications, we investigated systematically the death pathways of HeLa cells attached onto the drug model by means of a series of cell-biology techniques. It was demonstrated that the IFN-gamma plus TNF-alpha co-immobilized on the polymeric material surface exhibited more notable inhibitive effects than the free IFN-gamma plus TNF-alpha, and the induced HeLa cells were mainly along apoptosis-like PCD with the translocation of EndoG from the cytoplasm to the nucleus. These findings indicate that the polymeric drugs with the co-immobilized IFN-gamma plus TNF-alpha may offer significant potentials for therapeutic manipulation of human cervical cancer. (C) 2011 Elsevier Ltd. All rights reserved.
原文出处	://WOS:000289020600011
公开日期	2012-04-13
内容类型	期刊论文
源URL	[http://210.72.142.130/handle/321006/30364]
专题	金属研究所_中国科学院金属研究所
推荐引用方式 GB/T 7714	Y. Q. Guan,Z. B. Li,J. M. Chen,et al. Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs[J]. Biomaterials,2011,32(14):3637-3646.
APA	Y. Q. Guan.,Z. B. Li.,J. M. Chen.,H. M. Tao.,W. W. Wang.,...&J. M. Liu.(2011).Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.Biomaterials,32(14),3637-3646.
MLA	Y. Q. Guan,et al."Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs".Biomaterials 32.14(2011):3637-3646.