Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction

	Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction
	Bai HX ; Liu XH ; Yang F ; Yang XR ; Wang EK
刊名	chemical journal of chinese universities-chinese
	2010
卷号	31 期号:7 页码:1314-1321
关键词	AQUEOUS 2-PHASE SYSTEMS MASS-SPECTROMETRY IDENTIFICATION HUMAN PLASMA PROTEOME 2-DIMENSIONAL ELECTROPHORESIS SAMPLE PREFRACTIONATION CHROMATOGRAPHY FRACTIONATION MODEL PURIFICATION PARTITION
ISSN号	0251-0790
通讯作者	bai hx
中文摘要	for the first time, an aqueous two-phase system (atps) composed of n-propanol, nacl and water was applied in prefractionating proteomic proteins. in this work, ethanol, n-propanol and several commonly used inorganic salts were attempted to develop the atps. the atps composed of n-propanol, nacl and water was developed finally. subsequently, the effects of ph, salt concentration, n-propanol volume and protein concentration on the atps' separation effectiveness for proteins were studied. protein samples were prefractionated by the atps and then analyzed by gel electrophoresis. the results proved that the prefractionation method could separate proteomic proteins into three groups by one-step extraction, and there was no need for separating target proteins from the phase-forming reagents prior to gel electrophoresis analysis. simplicity, rapidity and low cost are the highlight attractiveness of the prefractionation method. it also has advantages of biocompability, potential for continuous operation, no requirement for sophisticated instrumentations and being easy or no need for separating the target proteins from phase-forming reagents prior to electrophoresis analysis. the prefractionation method has great significance in proteomics and methodology.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000280429500007
公开日期	2012-04-26
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43574]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Bai HX,Liu XH,Yang F,et al. Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction[J]. chemical journal of chinese universities-chinese,2010,31(7):1314-1321.
APA	Bai HX,Liu XH,Yang F,Yang XR,&Wang EK.(2010).Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction.chemical journal of chinese universities-chinese,31(7),1314-1321.
MLA	Bai HX,et al."Prefractionation Method to Separate Proteomic Proteins into Multigroups by One-step Extraction".chemical journal of chinese universities-chinese 31.7(2010):1314-1321.