Controlled Assembly of Protein in Glass Capillary
Lin YA ; Su ZH ; Balizan E ; Niu ZW ; Wang QA
刊名langmuir
2010
卷号26期号:15页码:12803-12809
关键词DIP-PEN NANOLITHOGRAPHY DROPLET EVAPORATION SURFACE ADSORPTION ARRAYS RESOLUTION PARTICLES GEOMETRY AFFINITY PATTERN
ISSN号0743-7463
通讯作者niu zw
中文摘要by means of a slow drying process and the control of surface charge characteristics, protein stripe patterns were readily prepared on the luminal surface of a capillary. we systematically studied the effects of surface properties, ph, and protein concentration on pattern formation using optical microscopy, atomic force microscopy, and quartz crystal microbalance measurement. by balancing these parameters, a broad selection of proteins could be assembled within a capillary with well-defined stripe patterns. neutravidin, one of the model proteins, was specifically chosen to demonstrate the bioactivity retained through the assembly process by interaction with fluorescently labeled biotin motifs. this technique therefore offers a facile approach for patterning proteins and other biomacromolecules in capillary tubes.
收录类别SCI收录期刊论文
语种英语
WOS记录号WOS:000280353800048
公开日期2012-04-26
内容类型期刊论文
源URL[http://ir.ciac.jl.cn/handle/322003/43564]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
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GB/T 7714
Lin YA,Su ZH,Balizan E,et al. Controlled Assembly of Protein in Glass Capillary[J]. langmuir,2010,26(15):12803-12809.
APA Lin YA,Su ZH,Balizan E,Niu ZW,&Wang QA.(2010).Controlled Assembly of Protein in Glass Capillary.langmuir,26(15),12803-12809.
MLA Lin YA,et al."Controlled Assembly of Protein in Glass Capillary".langmuir 26.15(2010):12803-12809.
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