Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface

	Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface
	Li XM ; Gao JQ ; Liu DJ ; Wang ZX
刊名	analytical methods
	2010
卷号	2 期号:8 页码:1008-1012
关键词	GOLD NANOPARTICLE PROBES PROTEIN MICROARRAYS SILVER ENHANCEMENT GLASS SLIDES NUCLEIC-ACID QUANTUM-DOT PARTICLE SYSTEMS BINDING ARRAYS
ISSN号	1759-9660
通讯作者	wang zx
中文摘要	here, a three-dimensional (3d) oligonucleotide microarray-based resonance light scattering (rls) assay has been developed for dna detection. oligonucleotide conjugated gold nanoparticles are used as rls probes for labeling the detection system, followed by silver deposition for signal enhancement. the 3d oligonucleotide microarray is fabricated on the home-made fourth-generation (g4) nh2-terminated poly(amidoamine) dendrimers (pamam) modified substrate. after activated by glutaraldehyde (ga), the pamam modified substrate enables to react with nh2-modified oligonucleotide with highly efficiency and generates highly signal-to-background ratio. this assay makes it possible to discriminate an oligonucleotide sequence from the target with single nucleotide mismatch with high selectivity. and detection sensitivity of the 3d microarray is nearly 3-fold higher than that of two-dimensional (2d) microarray which is fabricated on the commercial planar slide. the discrimination between g : t and c : t mismatch as well as the mismatch sites, a particularly difficult task for microarray-based assays, has also evaluated.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000281289400003
公开日期	2012-04-26
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/43508]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li XM,Gao JQ,Liu DJ,et al. Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface[J]. analytical methods,2010,2(8):1008-1012.
APA	Li XM,Gao JQ,Liu DJ,&Wang ZX.(2010).Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface.analytical methods,2(8),1008-1012.
MLA	Li XM,et al."Developing oligonucleotide microarray-based resonance light scattering assay for DNA detection on the PAMAM dendrimer modified surface".analytical methods 2.8(2010):1008-1012.