Effects of high-energy-pulse-electron beam radiation on biomacromolecules
Zhu, H ; Xu, JZ ; Li, SQ ; Sun, XY ; Yao, SD(姚思德) ; Wang, SL
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
2008
卷号51期号:1页码:86
ISSN号1006-9291
通讯作者Wang, SL (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
英文摘要To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by Co-60 gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future.
学科主题Computer Science; Engineering; Robotics
收录类别SCI
语种英语
WOS记录号WOS:000251972200013
公开日期2012-04-18
内容类型期刊论文
源URL[http://ir.sinap.ac.cn/handle/331007/7709]  
专题上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式
GB/T 7714
Zhu, H,Xu, JZ,Li, SQ,et al. Effects of high-energy-pulse-electron beam radiation on biomacromolecules[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2008,51(1):86.
APA Zhu, H,Xu, JZ,Li, SQ,Sun, XY,Yao, SD,&Wang, SL.(2008).Effects of high-energy-pulse-electron beam radiation on biomacromolecules.SCIENCE IN CHINA SERIES B-CHEMISTRY,51(1),86.
MLA Zhu, H,et al."Effects of high-energy-pulse-electron beam radiation on biomacromolecules".SCIENCE IN CHINA SERIES B-CHEMISTRY 51.1(2008):86.
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