Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA | |
Zhang, J(张炯) ; Lao, RJ ; Song, SP(宋世平) ; Yan, ZY ; Fan, CH(樊春海) | |
刊名 | ANALYTICAL CHEMISTRY |
2008 | |
卷号 | 80期号:23页码:9029 |
ISSN号 | 0003-2700 |
通讯作者 | Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
英文摘要 | In this work, we report a novel electrochemical DNA sensor based on a nonfouling monolayer structure self-assembled at gold surfaces. Self-assembled monolayers (SAMs) with oligo(ethylene glycol) (OEG)-terminated thiols are known to be highly protein-resistant and effectively repel nonspecific adsorption. We found that a mixed SAM structure incorporating thiolated oligonucleotides and OEG thiols (SH-DNA/OEG) exhibited the similar nonfouling feature. More importantly, it allowed facile electron transfer across the monolayer and thus was fully compatible with electrochemical detection. On the basis of this SH-DNA/OEG platform, we developed a sandwich-type electrochemical sensor for the sequence-specific detection of DNA targets. This sensor was able to detect as little as 1 pM target DNA even in the presence of complicated biological fluids such as human serum. We also employed this sensor to directly detect a polymerase chain reaction (PCR) amplicon from the genomic DNA of Escherichia coli K12, which led to a very low detect limit of 60 fg (similar to 10 copies). |
学科主题 | Physics |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000261335600070 |
公开日期 | 2012-04-18 |
内容类型 | 期刊论文 |
源URL | [http://ir.sinap.ac.cn/handle/331007/7688] |
专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
推荐引用方式 GB/T 7714 | Zhang, J,Lao, RJ,Song, SP,et al. Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA[J]. ANALYTICAL CHEMISTRY,2008,80(23):9029. |
APA | Zhang, J,Lao, RJ,Song, SP,Yan, ZY,&Fan, CH.(2008).Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA.ANALYTICAL CHEMISTRY,80(23),9029. |
MLA | Zhang, J,et al."Design of an Oligonucleotide-Incorporated Nonfouling Surface and Its Application in Electrochemical DNA Sensors for Highly Sensitive and Sequence-Specific Detection of Target DNA".ANALYTICAL CHEMISTRY 80.23(2008):9029. |
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