Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro

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	Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro
	Ni, J ; Wu, QY ; Li, YG(李宇国） ; Guo, ZX ; Tang, GS ; Sun, D ; Gao, F ; Cai, JM
刊名	JOURNAL OF NANOPARTICLE RESEARCH
	2008
卷号	10 期号:4 页码:643
ISSN号	1388-0764
通讯作者	Cai, JM (reprint author), Mil Med Coll 2, Dept Radiat Med, Shanghai 200433, Peoples R China
英文摘要	There is growing evidence in recent years that the pristine fullerene may be endowed with strong pro-oxidant capacity to biological samples. In this investigation we tested the hypothesis that water-soluble fullerene-C-60 (nano-C-60) may interact with ionizing radiation enhancing its antiproliferative effects. The two tumor cell lines with different radiosensitivity B16 and SMMU-7721 were treated by a combination of pristine fullerene and Co-60 gamma irradiation. We measured cell survival rates, apoptotic characteristics, reactive oxygen species (ROS) production and alteration of cell diameter with or without gamma-irradiation. There was reduced survival with B16 and SMMU-7721 cells exposed to nano-C-60, with the inhibitory concentrations reducing the viability by 50% to 65 part per billion (ppb) and 150 ppb respectively. For cells exposed to nano-C-60 prior to gamma-irradiation, damage to cell membranes and increased numbers of apoptotic cells were detected by morphologic Hoechst-staining analysis and Annexin V/propidium iodide double-staining. In cells exposed to nano-C-60, there were increased levels of ROS, as measured by fluorescence detection under laser confocal microscopy. Preincubation with non-toxic pristine C-60 before gamma-ray caused enlargement of cells with increased diameter. The results show that nano-C-60 inhibits the growth of tumor cells at certain concentrations and increases the effects of Co-60 gamma-irradiation, possibly through the elevated production of cellular ROS and the membrane disruption. Data in this study indicates a possible consideration of using C-60 as a candidate of sensitization modifier in tumor radiation biology.
学科主题	Spectroscopy
收录类别	SCI
语种	英语
WOS记录号	WOS:000253626900010
公开日期	2012-04-18
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7682]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Ni, J,Wu, QY,Li, YG,et al. Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro[J]. JOURNAL OF NANOPARTICLE RESEARCH,2008,10(4):643.
APA	Ni, J.,Wu, QY.,Li, YG.,Guo, ZX.,Tang, GS.,...&Cai, JM.(2008).Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro.JOURNAL OF NANOPARTICLE RESEARCH,10(4),643.
MLA	Ni, J,et al."Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro".JOURNAL OF NANOPARTICLE RESEARCH 10.4(2008):643.