Adenosine detection by using gold nanoparticles and designed aptamer sequences | |
Li, F ; Zhang, J ; Cao, XN ; Wang, LH(王丽华) ; Li, D(李迪) ; Song, SP(宋世平) ; Ye, BC ; Fan, CH(樊春海) | |
刊名 | ANALYST |
2009 | |
卷号 | 134期号:7页码:1355 |
ISSN号 | 0003-2654 |
通讯作者 | Wang, LH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
英文摘要 | Based on gold nanoparticles (AuNPs) and engineered DNA aptamers, we designed a novel bioassay strategy for the detection of adenosine as a small target molecule. In this design, an aptamer is engineered to consist of two pieces of random-coil like ssDNA which are respectively attached to AuNPs through their 50-thiol-modified end. They can reassemble into the intact aptamer tertiary structure and induce nanoparticle aggregation in the presence of the specific target. Results have demonstrated that gold nanoparticles can effectively differentiate these two different DNA structures via their characteristic surface plasmon resonance-based color change. With this method, adenosine can be selectively detected in the low micromolar range, which means that the strategy reported here can be applicable to the detection of several other small target molecules. |
学科主题 | Chemistry |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000267499800014 |
公开日期 | 2012-04-18 |
内容类型 | 期刊论文 |
源URL | [http://ir.sinap.ac.cn/handle/331007/7641] |
专题 | 上海应用物理研究所_中科院上海应用物理研究所2004-2010年 |
推荐引用方式 GB/T 7714 | Li, F,Zhang, J,Cao, XN,et al. Adenosine detection by using gold nanoparticles and designed aptamer sequences[J]. ANALYST,2009,134(7):1355. |
APA | Li, F.,Zhang, J.,Cao, XN.,Wang, LH.,Li, D.,...&Fan, CH.(2009).Adenosine detection by using gold nanoparticles and designed aptamer sequences.ANALYST,134(7),1355. |
MLA | Li, F,et al."Adenosine detection by using gold nanoparticles and designed aptamer sequences".ANALYST 134.7(2009):1355. |
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