The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator

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	The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator
	Zhang, LY(张兰勇） ; Wan, Y ; Zhang, J ; Li, D ; Wang, LH（王丽华） ; Song, SP（宋世平） ; Fan, CH（樊春海）
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2009
卷号	52 期号:6 页码:5
关键词	DNA detection conducting polymer HRP electrochemistry
ISSN号	1006-9291
通讯作者	Li, D (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
英文摘要	A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os(2+/3+) conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H(2)O(2), leading to a limit of detection as low as 10 fM.
学科主题	Chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000266168000007
公开日期	2012-04-11
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/7086]
专题	上海应用物理研究所_中科院上海应用物理研究所2004-2010年
推荐引用方式 GB/T 7714	Zhang, LY,Wan, Y,Zhang, J,et al. The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(6):5.
APA	Zhang, LY.,Wan, Y.,Zhang, J.,Li, D.,Wang, LH.,...&Fan, CH.(2009).The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(6),5.
MLA	Zhang, LY,et al."The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.6(2009):5.